不同碳、氮源与诱导物对球孢白僵菌产几丁质酶的影响

THE EFFECT OF VARIOUS CARBON AND NITROGEN SOURCES AND INDUCERS ON THE SYNTHESIS OF THE CHITINASE IN CULTURES OF BEAUVERIA BASSIANA

本实验通过研究各种不同碳、氮源及诱导物的作用,探求了提高白僵菌产几丁质酶活性的可能途径。一、材料和方法1.菌种球孢白僵菌（Beauveria bassiana 017）由吉林省农科院植保所提供。2.培养基 1)种子培养基:马铃薯葡萄糖琼脂斜面。2)基本培养基（g％）:MgSO4,KCI及K2HPO4均为0.075％,FeSO4为0.001％,自然pH值。

The maximum chitinase activity in cultures of Beauveria bassiana was observed when the fungi were grown in mediums containing ammonium sulphate 0.25%,peptone 0.5%,or lactic acid 0.2% separately and demineralized crab shell as inducer.