胶质瘤患者T淋巴细胞表面CTLA-4的表达及其临床意义

Expression of CTLA-4 in T lymphocytes of patients with glioma and its clinical significance

目的:探讨胶质瘤患者外周血中CD4^+和CD8^+T细胞表面CTLA-4表达与胶质瘤WHO分级及手术的关系,阐明CTLA-4的临床意义。方法:选择本院胶质瘤患者60例作为胶质瘤组,健康志愿者46名作为健康对照组。流式细胞术检测胶质瘤组和健康对照组研究对象外周血中CD4+和CD8^+T细胞表面CTLA-4的表达,分析其与胶质瘤WHO分级及手术的关系。结果:胶质瘤组患者外周血CD4^+和CD8^+T细胞表面CTLA-4的表达水平明显高于对照组(P<0.01);Ⅳ级胶质瘤患者CD4^+和CD8^+T细胞表面CTLA-4表达水平最高,Ⅳ级胶质瘤患者CD4^+和CD8^+T细胞表面CTLA-4表达水平高于Ⅲ级胶质瘤(P<0.01),Ⅲ级胶质瘤患者CD4^+和CD8^+T细胞表面CTLA-4表达水平高于Ⅱ级胶质瘤(P<0.01);术后胶质瘤患者CD4^+和CD8^+T细胞表面CTLA-4表达水平低于术前(P<0.01)。结论:胶质瘤患者外周血CD4^+和CD8^+T细胞表面CTLA-4的表达水平随着胶质瘤恶性程度的升高而升高,并在胶质瘤发生发展过程中发挥促进作用。

Objective： To investigate the correlation between the CTLA-4 expression in the peripheral blood CD4＋ and CD8＋ T cells in the patients with glioma and glioma WHO classification and operation, and to clarify the clinical significances of CTLA-4. Methods： 60 patients with gIioma from our hospital were selected as glioma group, and 46 healthy volunteers were used as control group. Then the CTLA-4 expression levels in the peripheral blood CD4＋ and CD8＋ T ceils of the subjects in glioma group and control group were detected by fluorescence-activated cell sorting （FACS） and the relationship between CTLA-4 and glioma WHO classification and surgery was analyzed. Results： The CTLA-4 expression levels in CD4＋ and CD8＋ T cells in peripheral blood of the patients in glioma group were higher than those in control group （P〈0.01）. The CTLA-4 expression level in the patients with grade IV glioma was the highest, the expression level of the patients with grade IV glioma was higher than that of the patients with grade HI glioma （P〈0.01）, and the expression level of grade Ill the patients with was higher than that of the patients with grade II （P〈0.01）. The expression level of CTLA 4 in CD4＋ and CD8＋T cells of the patients with glioma after operation was lower than before operation （P〈 0.01）. Conclusion： The CTLA-4 expression levels in the peripheral blood CD4＋ and CD8＋ T cells are increased with the increasing of malignancy degree of the patients with glioma, and it has a promotion role in the occurrence of development of glioma.