CDA基因单核苷酸多态性对中晚期非小细胞肺癌接受吉西他滨化疗患者预后的影响

Influence of single nucleotide polymorphisms of CDA in prognosis of advanced non-small cell lung cancer patients treated with gemcitabine

目的:探讨胞苷脱氨酶(CDA)基因单核苷酸多态性(SNPs)对吉西他滨化疗效果的影响,阐明CDA基因SNPs与晚期非小细胞癌(NSCLC)患者临床预后之间的关系。方法:收集93例经病理确诊后接受吉西他滨化疗的中晚期NSCLC初治患者,应用Sequenom Massarray Genotype系统检测CDA基因79A>C基因型。采用Kaplan-meier方法和Log-rank检验分析CDA基因79A>C SNPs与晚期NSCLC患者无进展生存期(PFS)以及总生存时间(OS)的关系,应用COX风险比例模型分析影响因素对预后的影响。结果:CDA基因79A>C基因多态性与NSCLC患者临床及肿瘤病理特征均未见明显相关性。携带野生型基因型A/A患者经吉西他滨化疗后中位PFS为9.3个月,而携带突变型基因型A/C和C/C患者中位PFS为11个月,二者比较差异无统计学意义(P=0.061)。携带野生型基因型A/A患者中位OS为21.7个月,携带突变型基因型A/C和C/C患者中位OS为22.1个月,二者比较差异无统计学意义(P=0.513)。结论:CDA基因79A>C SNPs对接受吉西他滨化疗的NSCLC患者的PFS有一定延长作用,未发现其SNP与OS有关联。

Objective; To investigate the influence of single nucleotide polymorphisms （SNP） of cytidine deaminase （CDA） in the curative effect of gemcitabine, and to clarify the correlation of CDA SNP with the clinical prognosis of the advanced non-small cell lung cancer （NSCLC） patients. Methods： CDA 79A〉C gene was detected by Sequenom Massarray Genotype System among 93 patients with advanced NSCLC who received chemotherapy of gemcitabine. The associations between CDA 79A〉C polymorphisms and progression free survival （PFS） and overall survival （OS） were estimated using Kaplan-meier methods and Log-rank test for univariate analysis, and COX proportional hazards model was used for multivariate analysis. Results： There was no significant correlation between the CDA 79A〉C SNPs with the clinical and pathological characteristics of NSCLC patients. The median PFS in the patients with wild genotype A/A was 9.3 months, while the median PFS in the patients with mutant genotype A/C and C/C was 11 months; there was no significant difference between them （P=0. 061）. The median OS in the patients with wild genotype A/A was 21.7 months, and the median OS of the patients with mutant genotype was 22. I months; there was no significant difference between them （P = 0. 513）. Oonclusion： CDA 79A〉C SNPs can prolong the PF$ of the NSCLC patients, but it does not affect the OS of the patients.