黄芪多糖对中波紫外线辐射皮肤角质形成细胞损伤的影响

Effects of Astragalus Polysaccharides on Damage of Keratinocytes in UVB Radiation Skin

目的观察黄芪多糖对中波紫外线(UVB)辐射损伤皮肤角质形成细胞(Ha Ca T细胞)的影响,初步探讨其作用机制。方法采用UVB辐射Ha Ca T细胞进行造模。分为空白对照组、模型组、阳性对照组及黄芪多糖低、中、高剂量组,各药物组给予相应药物干预。MTT法测定细胞活力;生化比色法检测谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量;ELISA检测肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量。结果与空白对照组比较,模型组SOD、CAT、GSH-Px含量降低,MDA、TNF-α、IL-6含量升高(P<0.05);与模型组比较,各药物组SOD、GSH-Px、CAT含量升高,MDA、TNF-α、IL-6含量降低(P<0.01)。结论黄芪多糖可在一定程度上减轻UVB对Ha Ca T细胞氧化应激性损伤。

Objective To investigate the effects of Astragalus polysaccharide (APS) on damage of keratinocytes in ultraviolet B (UVB) radiation skin in HaCaT cells; To preliminarily explore its mechanism of action.Methods HaCaT cells were treated by UVB irradiated for modeling. Blank control group, model group, UVB irradiation group and low-, medium- and high-dose APS interventional groups were set up. Each medication group was given relevant medicine. MTT assay was used to determine the cell activity; GSH-Px, CAT, SOD activity and MDA content were detected by biochemical colorimetric method; contents of TNF-α and IL-6 were detected by ELISA.Results Compared with the control group, the contents of SOD, CAT and GSH-Px in the model group decreased significantly, while the contents of MDA, TNF-α and IL-6 increased significantly (P<0.05); compared with the mode group, the contents of SOD, CAT and GSH-Px in the medication groups increased significantly, while the contents of MDA, TNF-α and IL-6 were reduced significantly (P<0.01).Conclusion APS can reduce the oxidative stress injury of UVB to HaCaT cells to some extent.