不同产地苦丁茶质量标准研究

Study on Quality Standard of Leaf of Chinese Holly from Different Habitats

目的建立苦丁茶的质量控制标准,为药用植物资源的开发和利用提供依据。方法收集10批不同产地苦丁茶样品,对苦丁茶性状进行描述,对苦丁茶叶的横切和粉末进行显微鉴别,以绿原酸为对照品对苦丁茶进行薄层鉴别,测定10批苦丁茶样品的水分、总灰分、酸不溶性灰分,采用高效液相色谱法对苦丁茶中绿原酸进行含量测定。结果对苦丁茶的性状、显微特征进行了描述,制订了苦丁茶中绿原酸的薄层色谱鉴别方法。检查项中暂订苦丁茶的水分不得超过13%,总灰分不得超过13%,酸不溶性灰分不得超过5%。确定了苦丁茶中绿原酸的含量测定方法,并根据测定结果暂订本品含绿原酸不得低于0.60%。结论本研究建立的方法可用于控制苦丁茶的质量。

Objective To set up the quality control standard for Leaf of Chinese Holly; To provide basis for the utilization and development of Leaf of Chinese Holly.Methods Ten batches of Leaf of Chinese Holly samples from different habitats were collected, and the properties were described. The crosscutting and powder of leaf was under microscopic identification. Chlorogenic acid was set as reference substance to conduct thin-layer identification. Moisture, total ash, and acid-insoluble ash of the 10 batches were detected. HPLC was used to detect chlorogenic acid in Leaf of Chinese Holly.Results The properties and microscopic identification were described. Thin-layer chromatography method for chlorogenic acid in Leaf of Chinese Holly was formulated. Check items temporarily required that the moisture in Leaf of Chinese Holly should be less than 13%, total ash less than 13%, and acid-insoluble ash less than 5%. Content determination method for chlorogenic acid in Leaf of Chinese Holly was confirmed. It temporarily required that the content of chlorogenic acid should be more than 0.60%.Conclusion The established method can be used for the formulation of quality control of Leaf of Chinese Holly.