龙藤筋骨片中蛇床子素含量测定研究

Study on content determination of osthole in Longtengjingu tablets

目的：建立龙藤筋骨片中蛇床子素的含量测定方法。方法：采用HPLC法测定龙藤筋骨片中蛇床子素的含量。选用welch Ultimate AQ-C18柱（4.6 mm×250 mm,5μm）;以乙腈-水（43∶57）为流动相;检测波长：330 nm;流速：1.5 m L·min-1;柱温：35℃。结果：HPLC法测定蛇床子素不存在干扰,蛇床子素进样量在1.560×10-3~0.046 8 mg·m L-1范围内时,线性关系良好（r=0.999 9）;平均回收率为100.82%,RSD为1.68%。结论：HPLC法测定蛇床子素含量简便、稳定、可靠,可作为龙藤筋骨片中蛇床子素的质量控制方法。

Objective： To establish a content determination method of osthole in Longtengjingu tablets. Methods：Thecontent of osthole in Longtengjingu tablets were determined by HPLC. The analysis was performed on welch Ultimate AQ-C18column （4.6 mm × 250 mm, 5 μm）. The mobile phase was consisted of acetonitrile and water （43 ： 57）. The detection wavelengthwas 330 nm. The flow rate was 1.5 mL·min-1. The column temperature was 35 ℃. Results： There was no interference for ostholedetection by HPLC method. The linear range of osthole was 1.560 × 10-3 – 0.046 8 mg·mL-1 （r = 0.999 9）. The average recovery was100.82% （RSD = 1.68%）. Conclusion： The method is simple, stable, reliable, and can be adopted as the quality control method of osthole.