SPE-GC/MS测定黄绿蜜环菌丙酮提取物脂溶性成分

Determination of Liposoluble Constituents in Armillaria Luteo-Virens Acetone Extract by SPE-GC/MS

为了研究黄绿蜜环菌子实体丙酮提取物的脂溶性成分。先用固相萃取(SPE)技术对黄绿蜜环菌子实体丙酮提取物进行预处理,再采用气相色谱-质谱(GC-MS)法对黄绿蜜环菌子实体丙酮提取物的脂溶性成分进行分析。共从黄绿蜜环菌子实体丙酮提取物中鉴定出33个化合物,占总提取物含量的98.8%。采用面积归一化法测定了各组分的百分含量,其中亚油酸含量高达48.2%。其余含量超过2%的成分依次为邻苯二甲酸丁辛酯(12.1%)、顺式-11-十八碳烯酸(7.1%)、邻苯二甲酸单(2-乙基己基)酯(7.3%)、棕榈酸(4.1%)、E-14-十六烯醛(3.3%)、E-3-二十烯(3.0%)、反式-13-十八碳烯酸(2.6%)、1-二十二烯(2.1%)。结果表明黄绿蜜环菌子实体丙酮提取物中富含脂肪酸类、酯类、烯类等化合物,为黄绿蜜环菌的进一步开发利用提供了依据。

To study liposoluble constituents of Armillaria luteo-virens acetone extract,solid phase extraction（SPE）technology was used to pretreat Armillaria luteo-virens acetone extract.Then,gas chromatography-mass spectrum（GC-MS）was used to analyze liposoluble constituents of Armillaria luteo-virens acetone extract.33 compounds were identified from Armillaria luteo-virens acetone extract,accounting for 98.8% of all extracts.Area normalization method was applied to determine percentage content of each constituent.The content of linoleic acid was as high as 48.2%,and the content of the following chemical components exceeds 2%：1-2-benzenedicarboxylic acid（12.1%）,（Z）-11-octadecenoic acid（7.1%）,phthalic acid mono-2-ethylhexylester（7.3%）,n-hexadecanoic（4.1%）,E-14-hexadecenal（3.3%）,（E）-3-eicosene（3.0%）,trans-13-octadecenoic acid（2.6%）and 1-nonadecene（2.1%）.The results indicate that,Armillaria luteo-virens acetone extract is rich in fatty acids,esters and alkenes.This study provides scientific reference and theoretic basis for further development and utilization of Armillaria luteo-virens.