乳腺癌中SRA1基因表达调控的生物信息学分析

Bioinformatic Analysis of SRA1 Gene in Breast Cancer

分析类固醇受体RNA激活物（steroid receptor RNA activator,SRA1）以长链非编码RNA形式（lncRNA SRA1）和蛋白质形式（SRA1蛋白）在乳腺癌中的分子调控网络。运用RegRNA、Targetscan、MicroCosm Targets、PicTar软件和HMDD、DAVID在线数据库,预测lncRNA SRA1与microRNA及下游靶基因间的多种调控途径,对靶基因进行功能富集分析,绘制lncRNA SRA1的核心调控网络图。同时运用TCGA数据库、string软件预测分析与SRA1蛋白相互作用的蛋白质。发现lncRNA SRA1上存在hsa-let-7b、hsa-let-7c、hsa-let-7g、hsa-let-7i、has-miR-92、has-miR-103、has-miR-194、has-miR-874、has-miR-141、has-miR-146a、has-miR-661这11个与乳腺癌相关microRNA的可能结合位点,从而调节下游230个靶基因,构建了lncRNA SRA1-miRNAs-mRNAs调控网络,提示lncRNA SRA1可能参与基因转录调控、生物合成、MAPK、癌症相关通路、紧密连接等信号通路。并且发现SRA1蛋白和DDX17、ESR1、NCOA2、NCOA1、NRIP1等多种蛋白存在相互作用。对SRA1基因调控网络的生物信息学分析有助于理解其在乳腺癌发生发展过程中的分子机制。

To predict the regulatory network of SRA1 both at RNA and protein level in breast cancer by the methods of bioinformatics.The RegRNA,Targetscan,MicroCosm Targets,PicTar bioinformatics applications and HMDD,DAVID databases were employed to predict the interaction between lncRNA SRA1 and microRNAs.At the same time,TCGA database and string application were utilized to develop the SRA1-centric proteins network.The potential binding sites of 11miRNAs（hsa-let-7b,hsa-let-7c,hsalet-7g,hsa-let-7i,has-miR-92,has-miR-103,has-miR-194,has-miR-874,has-miR-141,has-miR-146 a,has-miR-661）on lncRNA SRA1 sequence,and 230 target genes of the 11 miRNAs were found.The results indicated that the lncRNA SRA1 may play some parts in transcription,biosynthetic process,MAPK signaling pathway,cancer related pathways and tight junction.In addition,it also showed SRA1 protein interacted with multitude proteins.It was demonstrated that the regulatory network of SRA1 by bioinformatics provided reliable clues for exploring the molecular mechanisms of SRA1 gene in breast cancer.