粒度和加水量对厚朴煮散煎出物的影响

Effect of Particle Size and Water Amount on Decoction of Magnoliae Officinalis Cortex Boiling Powder

目的:优选厚朴煮散的粉碎粒度,考察加水量对其煎出物的影响,为该煮散的临床应用提供参考。方法:制备5种规格的厚朴煮散,采用HPLC检测水煎液中厚朴酚、和厚朴酚含量,检测波长294 nm,流动相甲醇-水(82∶18),柱温30℃。以厚朴酚、和厚朴酚转移率和干膏率为指标,通过单因素试验考察粉碎粒度和加水量对厚朴煮散煎出物的影响,并与饮片传统煎煮进行比较。结果:粒度和加水量对厚朴煮散水煎液有效成分的转移率和干膏率均有显著影响。厚朴煮散最佳制备工艺为粉碎过10目筛,含有能通过80目筛的细粉不超过20%;厚朴煮散煎煮1次,煎煮时间10 min时,24目煮散加20倍量水的煎液中有效成分转移率和得膏率分别为饮片传统煎煮(加10倍量水煎煮50 min)的5.1,2.5倍;10目煮散加10倍量水的煎液中二者分别达饮片传统煎煮的2倍以上。结论:厚朴饮片制成煮散后可节省药材和煎煮时间,可推广于临床中应用。

Objective： To optimize partical size of Magnoliae Officinalis Cortex boiling powder,and investigate effect of the amount of water on decoction of this boiling powder. Method： Five kinds of particle size of Magnoliae Officinalis Cortex boiling powder were prepared. Contents of magnolol and honokiol in decoction were determined by HPLC,mobile phase consisted of methonl-water（ 30 ∶ 70） with a flow rate of 1. 0 m L·min- 1and detection wavelength was at 294 nm. With transfer rates of magnolol and honokiol,dry extract rate as indexes,effects of particle size and the amount of water on decoction of Magnoliae Officinalis Cortex boiling powder were investigated by single factor tests,and compared with traditional decoction of herbal pieces. Result： Particle size and water amount both had significant effect on compound content and dry extract rate in decoction of this boiling powder. Optimum preparation process was that particles must be all through 10 mesh sieve,and fine powder of 80 mesh sieve shall not exceed 20%. When boiling powder was decocted once with decoction time of 10 min,transfer rate of compound and dry extract rate of boiling powder（ particles must be all through 24 mesh sieve,added 20 times the amount of water） were successively 5. 1 and 2. 5 times those in traditional decoction of herbal pieces（ added 10 times the amount of water,decoction time of 50 min）,those of boiling powder（ particles must be all through 10 mesh sieve,10 times the amount of water） were all 2 times those in traditional decoction of herbal pieces. Conclusion： Boiling powder of Magnoliae Officinalis Cortex boiling powder can save herbs and decoction time by comparing with traditional herbal pieces.