基于美白功效对当归补血汤药效关系的分析

Analysis on Pharmacodynamic Relationship of Danggui Buxue Tang Based on Whitening Efficacy

目的:通过研究当归补血汤中黄芪、当归不同配比对美白功效的影响,探索该复方美白功效的最佳药效配比。方法:采用生化酶学法测定当归补血汤对酪氨酸酶的抑制率1,1-二苯基-2-三硝基苯肼(DPPH)分析法测定当归补血汤的抗氧化活性,综合2种方法筛选当归补血汤的美白功效的最佳药效配比。采用Franz扩散池试验评价该复方的透皮吸收效果。结果:当归补血汤的配伍最佳剂量组合为黄芪-当归(1∶7),对酪氨酸酶活性的抑制率89.65%,对DPPH自由基的清除率90.96%。阿魏酸和毛蕊异黄酮葡萄糖苷的24 h累积透过量分别为0.030 4,0.001 2 mg·cm-2,渗透率依次为59.27%,57.79%。结论:方剂配伍比例的变化会影响其功效的发挥,甚至改变组成方中药物的地位,使全方主治证及功用发生变化,为当归补血汤开发成为绿色天然美白化妆品提供参考。

Objective： To explore the best ratio of Danggui Buxue Tang for whitening efficacy by investigating whitening efficacy caused by different proportion of Astragali Radix and Angelicae Sinensis Radix in this compound. Method： Biochemical-enzymic method was adopted to measure tyrosinase inhibition rate of Danggui Buxue Tang and 1,1-diphenyl-2-picrylhydrazyl（ DPPH） method was adopted to measure its antioxidant activity. Franz diffusion cell test was used to evaluate skin permeation of Danggui Buxue Tang. Result： Optimum proportion of Astragali Radix and Angelicae Sinensis Radix in this compound was 1 ∶ 7. Under this proportion,its inhibition rate of tyrosinase achieved 89. 65% and scavenging ratio of DPPH free radicals achieved 90. 96%.Accumulative permeation amounts in 24 h of ferulic acid and calycosin-7-O-β-D-glucoside were 0. 030 4,0. 001 2mg·cm- 2,permeabilities were 59. 27% and 57. 79%,respectively. Conclusion： Change of compatibility ratio can affect efficacy of Danggui Buxue Tang,or even change status of compatibility between monarch and ministerial drug,then it may change syndrome and function of this prescription.