不同促渗剂对三威跌打风湿贴中柚皮苷体外透皮吸收的影响

Effects of Different Permeation Enhancer on in Vitro Percutaneous Absorption of Naringin in Sanwei Dieda Rheumatism Gel Paste

目的:研究三威跌打风湿贴中柚皮苷体外透皮吸收行为,比较不同透皮吸收促进剂对其透皮吸收性能的影响。方法:采用Franz扩散池装置,以HPLC测定接收液中柚皮苷含量,流动相0.1%磷酸-乙腈(81∶19),检测波长283 nm,考察制剂中柚皮苷体外透皮吸收行为及各促透剂的促渗效果。结果:不含促渗剂的凝胶膏剂中柚皮苷体外透皮吸收符合零级释放模型,24 h体外累积渗透方程Q=1.126t+2.795,透皮速率1.126μg·cm-2·h-1;加入促透剂丙二醇、氮酮、薄荷脑后柚皮苷体外透皮吸收速率明显提高,且薄荷脑>丙二醇>氮酮。结论:凝胶膏剂中柚皮苷可透皮吸收;促透剂丙二醇、氮酮、薄荷脑均能促进柚皮苷透皮吸收,且以薄荷脑作用最明显。

Objective： To investigate effects of different permeation enhancer on in vitro percutaneous absorption of naringin in Sanwei Dieda rheumatism gel paste. Method： Taking Franz diffusion cell to perform test,HPLC was adopted to determine the content of naringin in receiving liquid,effects of different permeation enhancer on in vitro percutaneous absorption of naringin in Sanwei Dieda rheumatism gel paste were investigated. Result： In vitro percutaneous absorption of naringin in this gel paste accorded with zero order release model, cumulative permeation equation was Q = 1. 126 t ＋ 2. 795 with transdermal rate of 1. 126 μg·cm- 2·h- 1. Either propylene glycol,azone or menthol was added, transdermal rates of naringin were improved, and effect was in order of menthol 〉 propylene glycol 〉 azone. Conclusion： Naringin in gel preparation can be percutaneous absorded.Propylene glycol,azone and menthol all can obviously promote transdermal absorption of naringin,especially for menthol,which plays the highest effect.