双黄连注射液及其中间品对RBL-2H3细胞增殖的影响

Effect of Shuanghuanglian Injection and Its Intermediates on Proliferation of RBL-2H3 Cells

目的:探讨双黄连注射液(SHLI)及其中间品(金银花连翘提取物、黄芩提取物)不同浓度对白血病细胞株RBL-2H3增殖和形态的影响,为类过敏细胞模型的建立及药品质量的监控提供实验依据。方法:将双黄连注射液以体积比1∶16为初始浓度,按2倍梯度稀释至1∶4 096,中间品分别以体积比1∶8为初始浓度,按2倍梯度稀释至1∶2 048,作为受试样品。以各受试样品分别孵育RBL-2H3细胞,观察细胞形态变化。应用噻唑蓝(MTT)比色法测定各组RBL-2H3细胞增殖活力,并计算细胞增殖的半数抑制浓度(IC50)。根据IC50设置不同给药浓度,观察SHLI对RBL-2H3细胞凋亡的影响。结果:随着给药浓度的升高,SHLI及其中间品对细胞增殖的抑制作用逐渐增强,细胞存活力明显降低。给药后随着药物浓度的升高,细胞损伤逐渐加重,形态发生明显变化。高浓度SHLI可引起RBL-2H3细胞凋亡。结论:SHLI可抑制RBL-2H3细胞增殖活力,为SHLI类过敏细胞模型的建立,评价SHLI及中间品的安全性及药物质量控制提供依据。

Objective： To observe the effect of different concentrations of Shuanghuanglian injection（ SHLI） and its intermediates on proliferation of RBL-2H3 cells, and provide evidence for establishing anaphylactoid cell models and monitoring drug quality. Method： RBL-2H3 cells were treated with different concentrations of SHLI（ 2 times gradient dilution from volume ratio 1∶16 to 1∶4 096） and its intermediates（ 2 times gradient dilution from volume ratio 1 ∶ 8 to 1 ∶ 2 048）. The cellular morphology was observed under inverted microscope. MTT colorimetric assay was used to determine the growth of RBL-2H3 cells in different concentration of SHLI and its intermediates,and the half inhibitory concentration（ IC50） was calculated. Dependent on the value of IC50,RBL-2H3 cells were then treated with different concentrations of SHLI to observe the effect of SHLI on cells apoptosis. Result： As the concentration increases of SHLI and its intermediates, their inhibitory effect on proliferation of RBL-2H3 cells was gradually increased; the cells survival rate was decreased significantly; cells injury was gradually aggravated; and the cellular morphology was also changed. High dose of SHLI could induce apoptosis of RBL-2H3 cells. Conclusion： SHLI and its intermediates could reduce RBL-2H3 cells proliferation,we can provide certain experiment basis for establishing anaphylactoid cell models and monitoring drug quality and safety.