益气化瘀方对血瘀证子宫复旧不全模型大鼠血液高凝状态的影响

Effect of Yiqi Huayu Decoction on Hypercoagulable State in Rat Models of Blood Stasis Syndrome with Uterus Subinvolution

目的：观察产后子宫复旧不全大鼠血液流变学和组织纤溶酶原激活物（t-PA）的含量变化,探讨血瘀证子宫复旧不全大鼠模型的构建及益气化瘀方对其干预机制。方法：50只妊娠大鼠被随机分为5组,分别为正常组、模型组、新生化组（5.4 g·kg-1）及益气化瘀低、高剂量组（4.68,18.72 g·kg-1）。各组产后予以大肠埃希菌宫腔内给药制备炎症产后子宫复旧不全模型（正常组不造模）,并于造模后第1-7天分别ig给药,子宫组织常规苏木素-伊红（HE）染色观察子宫组织形态。采用血液流变仪测定血流变指标,采用双抗体夹心法测定血清t-PA的含量。结果：与正常组比较,模型组全血黏度和血浆黏度明显升高（P〈0.05）;与模型组比较,新生化组、益气低、高剂量组明显降低全血黏度和血浆黏度（P〈0.05）,益气高剂量组优于新生化组（P〈0.05）。模型组中t-PA水平高于正常组（P〈0.05）;新生化组、益气低、高剂量组中t-PA水平较模型组降低（P〈0.01）,且各组间比较无统计学意义。结论：子宫内膜炎所导致的产后子宫复旧不全大鼠模型满足血瘀证动物模型要求。益气化瘀方能改善产后血液的黏滞状态,加速血液循环,促进产后余血的排出。同时它通过降低t-PA高水平,降低血液黏滞程度,抑制瘀血形成,促进新血再生,帮助产后子宫复旧。

Objective： To observe the hemorrheology and tissue plasminogen activator（ t-PA） changes in uterus subinvolution rats after delivery,and explore the construction of uterus subinvolution rat models and intervention mechanism of Yiqi Huayu decoction. Method： Fifty pregnant rats were randomly divided into 5groups： normal group,model group,Xinshenghua group（ 5. 4 g·kg- 1）,and Yiqi Huayu low dose group and high-dose group（ 4. 68,18. 72 g ·kg- 1）. Rats in all other groups except normal group received postpartum intrauterine administration of Escherichia coli to prepare inflammation postpartum uterussubinvolutionmodels. All rats were administered by intragastricinfusion with corresponding drugs during day 1-7 after the models were established. HE staining of the uterus tissues was done to observe the morphology of uterus. Blood rheometer was used to detect the hemorrheology index and double antibody sandwich method was used to detect tissue plasminogen activator（ t-PA） levels. Result： Compared with the normal group,whole blood viscosity and plasma viscosity were significantly increased in model group（ P〈 0. 05）. Compared with model group,whole blood visosity and plasma viscosity were significantly decreased in Xinshenghua group and low-,high-dose Yiqi Huayu groups（ P 0. 05）,and the effect in Yiqi Huayu high-dose group was better than that in Xinshenghua group（ P〈 0. 05）. The level of t-PA in model group was higher than that in normal group（ P〈 0. 05）. Levels of t-PA were decreased in Xinshenghua group and Yiqi Huayu low and high dose groups（ P〈 0. 01）,but there was no statistically significant difference between various treatment groups. Conclusion： Postpartum uterus subinvolution rat models induced by endometritis meet the requirements of blood stasis syndrome animal models. Yiqi Huayu decoction could improve the viscous state of postpartum blood,accelerate blood circulation,and promote postpartum blood discharge. It also could reduce the high levels of t-PA,decrease the blood viscosity degree,inhibit the formation of new blood stasis,promote regeneration of blood,and help postpartum uterine involution.