杜仲丸不同配比对去卵巢大鼠骨质疏松症的影响

Effect of Duzhong Wan with Different Proportions on Osteoporosis in Ovariectomized Rats

目的:观察杜仲、续断不同配比组成的杜仲丸对去卵巢大鼠骨质疏松症的影响。方法:通过切除3月龄SPF级SD雌性大鼠双侧卵巢的方法建立绝经后骨质疏松症大鼠模型。造模4周后,将70只模型大鼠随机分为模型组,雌激素组(E_2组),杜仲、续断不同比例杜仲丸组,杜仲与续断生药比分别为2∶1,1∶1,1∶2,1∶0,0∶1。造模时仅切除部分卵巢周围脂肪组织的大鼠为假手术组,分别ig给予相应药物或蒸馏水。治疗干预12周后,检测各组大鼠骨密度(BMD),骨微结构参数,大鼠血清雌激素雌二醇(E_2),骨形成蛋白-2(BMP-2),大鼠Ⅰ型胶原C端肽(CTX-Ⅰ)含量。结果:与假手术组比较,模型组子宫系数,右股骨系数,BMD,骨体积分数(BV/TV)及血清E_2含量均显著下降,而血清BMP-2,CTX-Ⅰ水平显著升高(P<0.01,P<0.05)。与模型组比较,E_2组和1∶1组的子宫系数,E_2组,2∶:1组,1∶1组,1∶2组,1∶0组的右股骨系数,BMD,E_2组,2∶1组和1∶1组BV/TV均显著升高;2∶1组,1∶1组,1∶0组的血清BMP-2含量,E_2组,2∶1组,1∶1组,1∶2组,1∶0组的血清CTX-I显著降低(P<0.01,P<0.05)。结论:杜仲、续断不同配比组成的杜仲丸对于绝经后骨质疏松症均具有治疗作用,但是治疗作用存在一定差异。

Objective： To study the effect of Duzhong Wan with different proportions on the osteoporosis in ovariectomized rats. Method： Post menopausal osteoporosis（ PMOP） rat models were established by bilateral ovariectomy of 3-month-old SD female rats. 4 weeks after successful modeling,70 model rats were randomly divided into the model group,estrogen group（ E_2group）,Eucommiae Cortex and Dipsaci Radix（ with different proportions） groups（ 2∶ 1,1∶ 1,1∶ 2,1∶ 0,0∶ 1）. The rats with only removal of part fat tissues around the ovary in modeling were used as sham operation group. Corresponding drugs and distilled water were given by ig administration to the rats. Bone mineral density（ BMD）,bone microstructure parameters,serum estradiol（ E_2）,bone morphogenetic protein 2（ BMP-2） and c-terminal peptide of collagen type Ⅰ（ CTX-Ⅰ） levels were detected respectively after treatment 12 weeks. Result： Compared with sham operation group,uterine coefficient,right femur coefficient,BMD,bone volume fraction（ BV / TV）,and serum estrogen levels were significantly decreased in model group,but the level of BMP-2 and CTX-Ⅰin serum were significantly increased（ P〈 0. 01,P〈 0. 05）.Compared with the model group,uterine coefficient in E_2 group and 1∶ 1 group,right femur coefficient and BMD in E_2 group,2∶ 1 group,1∶ 1 group,1∶ 2 group and 1∶ 0 group,as well as BV / TV in E_2 group,2∶ 1 group,and1∶ 1 group were significantly increased; serum BMP-1 levels in 2∶ 1 group,1∶ 1 group and 1∶ 0 group,and serum CTX-I levels in E_2 group,2 ∶ 1 group,1∶ 1 group,1 ∶ 2 group and 1 ∶ 0 group were significantly decreased（ P 〈0. 01,P〈 0. 05）. Conclusion： The results suggested that Duzhong Wan with different proportions of Eucommiae Cortex and Dipsaci Radix had treatment effect for post menopausal osteoporosis,while there may be a difference in intensity of the effect.