(E)-苯乙基-3-(3,5-二羟基-4-异丙基苯基)丙烯酸酯对小鼠皮炎湿疹的治疗作用初步研究

The therapeutic effect of(E)-phenethyl 3-(3, 5-dihydroxy-4-isopropylphenyl) acrylate gels on dermatitis eczema in mice and the mechanism

目的研究(E)-苯乙基-3-(3,5-二羟基-4-异丙基苯基)丙烯酸酯(THCA354)凝胶剂对小鼠变应性接触性皮炎(ACD)的治疗作用及机制。方法通过对小鼠耳廓涂抹2,4-二硝基氟苯(DNFB)诱导建立ACD模型,测定给予DNFB刺激后24 h的耳肿胀厚度和对小鼠耳组织进行病理组织形态学分析来共同评价THCA354凝胶剂的抗过敏作用效果;采用ELISA法和RT-PCR定量检测小鼠耳组织中炎症细胞因子含量及m RNA表达水平,初步阐明其作用机制。结果与阴性对照组相比,局部涂抹THCA354的小鼠耳肿胀程度、淋巴细胞浸润程度、耳组织匀浆中促炎细胞因子含量和m RNA表达水平均显著降低,抗炎细胞因子的含量和m RNA表达水平均显著升高。结论 THCA354可能是通过改变炎症细胞因子的分泌和m RNA表达水平,调节T细胞亚群的平衡从而发挥抗过敏作用。

The present study was designed to investigate the therapeutic effects of（E）-phenenthyl-3-（3,5-dihydroxy-4-isopropyl phenyl） acrylate gels（THCA354） on mouse allergic contactdermatitis（ACD） and toexplore the probable mechanisms. 1-fluoro-2, 4-dinitrobenzene（DNFB） was daubed on the ear of mice to induceACD model. Twenty-four hours after DNFB stimulation, by measuring the thickness of the ear and analyzing thehistopathologic morphology of ear tissue, we evaluate the anti-allergic effect of THCA354 gels. The protein andm RNA expression levels of inflammatory cytokines in mouse ear tissue homogenate were determined by ELISA andRT-PCR method. Compared with negative vehicle, the ear thickness, invasion degree of lymph cell and the proteinand m RNA levels of proinflammatory cytokine in ear tissue homogenate were significantly reduced, andanti-inflammatory cytokines were significantly raised in the group locally treated with THCA354 gel. In conclusion,THCA354 gels can exert anti-allergic effects by regulating the secretion and im RNA expression of inflammatorycytokines, thus to regulate the balance of the T cells subgroup.