植物内生链霉菌KIB-271抗植物病原真菌活性成分研究

Secondary Metabolites from an Endophytic Streptomyces sp.KIB-271 and Their Anti-fungus Activity

利用硅胶柱层析和制备型高效液相色谱等方法从来自杜鹃花科植物的一株内生链霉菌Streptomyces sp.KIB-271的发酵液中分离得到4种戊二酰亚胺类化合物,通过分析其理化性质和波谱数据,分别鉴定为cycloheximide(1)、iso-cycloheximide(2)、2,6-二酮-4-[(E)-2'-((3″S,5″S)-3″,5″-二甲基-2″-酮-环亚己基)]-乙基-哌啶(3)和2,6-二酮-4-[(E)-2'-((3″R,5″S)-3″,5″-二甲基-2″-酮-环亚己基)]-乙基-哌啶(4),其中,4为首次从天然产物中分离得到。抗菌试验研究表明化合物1对多株植物病原真菌具有明显的抗菌活性,可能是Streptomyces sp.KIB-271代谢产物的主要活性物质之一。

Four heterecyclic glntarimide antibiotics, namely eycloheximide （ 1 ）iso-eycloheximide （ 2 ）, 4- [ 2＇- （ 3＂ （S）, 5＂（S） -3＂,5＂-dimethyl-2＂-oxocyclohexylidene） ethyl] piperidine-2,6-dione （3）, and 4-[ 2＇-（3＂（R） ,5＂（S） -3＂,5＂-dime- thyl-2＂-oxocyclohexylidene） ethyl] piperidine-2,6-dione （4） ,were isolated from the endophytic actinomycete Streptomy- ees sp. KIB-271. Their structures were identified through mass spectrometry and NMR experiments. Among them, com- pound 4 was reported for the first time as a natural product. In the antimicrobial experiments, 1 exhibited obvious antifun- gal activity in vitro against most of the tested fungus, and was considered as one of the main antimierobial constituent from Streptomyees sp. KIB-271.