大苞短毛唇柱苣苔离体培养和快速繁殖

In vitro Culture and Rapid Propagation of Chirita brachytricha.var.magnibracteata W.T.Wang et D.Y.Chen

为了探索大苞短毛唇柱苣苔离体培养和快速繁殖的最佳培养条件。以大苞短毛唇柱苣苔叶片为外植体,建立其组培快繁再生体系。结果表明大苞短毛唇柱苣苔叶片外植体的最优消毒方法为:采用75%酒精灭菌15 s,0.1%Hg Cl2灭菌4 min;叶片诱导不定芽最佳培养基为:MS+TDZ0.5 mg/L+NAA0.1 mg/L,诱导率为83.75%;不定芽增殖最佳培养基为MS+6-BA0.5 mg/L+NAA0.1 mg/L,增殖系数可达16,且不定芽生长良好;生根培养基以1/2MS+IBA0.1 mg/L为最好,生根率达96.67%以上。组培苗经炼苗及移栽,成活率达95%以上。建立了大苞短毛唇柱叶片组织培养的快繁体系,为规模化生产大苞短毛唇柱苣苔提供技术指导。

In this study, the condition of in vitro culture and rapid propagation of Chirita brachytricha, var. magnibracte- ata W. T. Wang et D. Y. Chen was investigated with its leaves as explants. The effects of different hormones for callus in- duction and plant regeneration of leaves were studied. The results showed that：the optimal way to obtain sterile explant for leaves were sterilized in 75% ethyl alcohol forl5 s then 0. I% HgC12 for 4 min;The optimal medium for callus of leaves was MS ＋ TDZ0.5 mg/L ＋ NAA0. 1 mg/L,induction rate was 83.75% ;The optimal medium of induction of ad- ventitious buds was MS ＋6-BA0.5 mg/L ＋ NAA0.1 mg/L,Muhiplication factor was 16,and seedlings grew well;The optimal medium and plant growth substances combination for rooting induction was 1/2MS ＋ IBA0.1 mg/L;Rooting rate was 96.67%. Transplant survival rate of plantlet reached more than 95%. In conclusion,the condition of plant regenera- tion and clonal propagation system was established. It provided technical guidance for production of C. brachytricha, var. magnibracteata W. T. Wang et D. Y. Chen.