摘要
为了探索大苞短毛唇柱苣苔离体培养和快速繁殖的最佳培养条件。以大苞短毛唇柱苣苔叶片为外植体,建立其组培快繁再生体系。结果表明大苞短毛唇柱苣苔叶片外植体的最优消毒方法为:采用75%酒精灭菌15 s,0.1%Hg Cl2灭菌4 min;叶片诱导不定芽最佳培养基为:MS+TDZ0.5 mg/L+NAA0.1 mg/L,诱导率为83.75%;不定芽增殖最佳培养基为MS+6-BA0.5 mg/L+NAA0.1 mg/L,增殖系数可达16,且不定芽生长良好;生根培养基以1/2MS+IBA0.1 mg/L为最好,生根率达96.67%以上。组培苗经炼苗及移栽,成活率达95%以上。建立了大苞短毛唇柱叶片组织培养的快繁体系,为规模化生产大苞短毛唇柱苣苔提供技术指导。
In this study, the condition of in vitro culture and rapid propagation of Chirita brachytricha, var. magnibracte- ata W. T. Wang et D. Y. Chen was investigated with its leaves as explants. The effects of different hormones for callus in- duction and plant regeneration of leaves were studied. The results showed that:the optimal way to obtain sterile explant for leaves were sterilized in 75% ethyl alcohol forl5 s then 0. I% HgC12 for 4 min;The optimal medium for callus of leaves was MS + TDZ0.5 mg/L + NAA0. 1 mg/L,induction rate was 83.75% ;The optimal medium of induction of ad- ventitious buds was MS +6-BA0.5 mg/L + NAA0.1 mg/L,Muhiplication factor was 16,and seedlings grew well;The optimal medium and plant growth substances combination for rooting induction was 1/2MS + IBA0.1 mg/L;Rooting rate was 96.67%. Transplant survival rate of plantlet reached more than 95%. In conclusion,the condition of plant regenera- tion and clonal propagation system was established. It provided technical guidance for production of C. brachytricha, var. magnibracteata W. T. Wang et D. Y. Chen.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第3期350-353,共4页
Natural Product Research and Development
基金
贵州省科技厅推广项目(2012-5033)
关键词
大苞短毛唇柱苣苔
离体培养
快速繁殖
Chirita brachytricha, var. magnibracteata W. T. Wang et D. Y. Chen
in vitro culture
rapid propagation