颞叶癫痫小鼠外周血细胞因子mRNA水平变化

Cytokines expression and significance in the peripheral blood from mice with temporal lobe epileptic in the acute phase

目的研究颞叶癫痫小鼠发作2 h外周血IFN-α6、IFN-γ、IL-1β以及TNFR-m18等细胞因子变化情况。方法采用real-time PCR法检测癫痫发作后10 min、30 min、1 h和2 h癫痫小鼠外周血中IFN-α6、IFN-γ、IL-1β以及TNFR-m18 m RNA的表达。结果癫痫组30 min时IL-1βm RNA的表达水平与对照组及SE10 min比较均升高(P<0.05);癫痫组10 min时IFN-α6 m RNA表达水平低于对照组(P<0.05);IFN-γm RNA表达水平在SE10 min时与对照组及SE其他时间点相比有统计学差异(P<0.05);TNFR-m18 m RNA表达水平在SE 1 h时与对照组及SE其他时间点(10 min、30 min、2 h)相比升高(P<0.01)。结论癫痫状态下外周血中细胞因子m RNA表达水平失衡,可能与急性期癫痫的免疫病理过程有关。

Objective To study the expression changes of IL-1β, FN-α6, IFN-γ and TNFR-m18 in peripheral blood within 2 hours in epileptic mice. Methods Semi quantitative real-time PCR was used to test the mRNA expres- sion level of IL-1β, FN-α6, IFN-γ, and TNFR-m18 in peripheral blood from normal and pilocarpine-induced epileptic mice at different time points （10 min, 30 rain, 1 h and 2 h）. Results The mRNA expression level of IL-1β increased at 30 rain （1.8±0.07）, 1 h （ 2.9±0.98 ） and 2 h （ 1.45±0.11 ） after pilocarpinc-induced status epilepticus comparing with that of control and SE 10 min（0.81±0.09）（P〈0.05）. The IFN-α6 mRNA expression level was lower at 10 min（0.59±0.05, P〈 0.05） than that of control. IFN-γmRNA expression level was higher at 10 min（2.85±0.11） than that of control and at oth- er time points during SE （P〈0.01）. TNFR-m18 mRNA expression leve] was higher at l h （2.84±0.15） than that of control, and at other time points during SE（P〈0.01 ）. Conclusion The immune system of epileptic state is active, the imbalance of cytokine expression in peripheral blood may be related to the immune pathological process of acute stage of epilepsy.