HPLC测定丹芩凉血丸中黄芩苷、丹皮酚、黄芩素和汉黄芩素的含量

Determination of Baicalin, Paeonol, Baicalein and Wogonin in Danqin Liangxue Pills by HPLC

目的建立高效液相色谱法同时测定丹芩凉血丸中黄芩苷、丹皮酚、黄芩素和汉黄芩素含量的方法。方法采用Waters XTerra RP18色谱柱（4．6mm×250mm；5μm）；流动相为甲醇-0．05％磷酸溶液，梯度洗脱；检测波长为274nm；流速为1．0mL／min；柱温35℃结果黄芩苷、丹皮酚、黄芩素和汉黄芩素分别在0．0078～0．4710，0．0091～0，5448，0．0498～2.9904和W0．0098～0．5850gg有良好的线性关系，平均回收率分别为98．78％，98．81％，98．30％和100．40％，RSD分别为1．67％，1．81％，1．20％和1．27％。结论本法简便、快速、结果准确、重复性好，可用于丹芩凉血丸的质量控制。

Objective To establish an HPLC method for the simultaneous determination of baicalin, paeonol, baicalein and wogonin in Danqin Liangxue Pills. Methods Baicalin, paeonol, baicalein and wogonin were separated on a Waters XTerra RP18 （4.6 mm×250 mm, 5 μm） column. Gradient elution was performed, with methanol-0.05 % phosphate solution as mobile phase. The detection wavelength was 274 nm and the flow rate was 1.0 mL/min. The column temperature was 35 ℃. Results The linear ranges of baicalin, paeonol, baicalein and wogonin were 0.0078-0.4710, 0.0091-0.5448, 0.0498-2.9904 and 0.0098-0.5850 μg, and the average recoveries were 98.78 %, 98.81%, 98.30 % and 100.40 %, respectively, with RSDs of 1.67 %, 1.81%, 1.20 % and 1.27 %, respectively. Conclusion The method can be convenient, quick, accurate and reproducible. It can be used for the quality control of Danqin Liangxue Pills.