循环CD34^+细胞数量与老年高血压的关系

Correlation between circulating CD34^+ level and elderly patients with hypertension

目的探讨循环CD34^+细胞水平与老年高血压关系,分析Ang-1/Tie-2在内皮祖细胞的表达及其调控意义。方法选择老年高血压患者59例为高血压组,正常健康者59例为对照组。RT-PCR法检测Ang-1/Tie-2 mRNA表达,应用流式细胞仪测定外周血CD34^+/PMNC值。分析外周血CD34^+/PMNC值与各临床指标的相关性,以及Ang-1和Tie-2 mRNA水平与CD34^+/PMNC值的相关性。结果高血压组外周血CD34^+/PMNC值显著低于对照组(P=0.000)。高血压组外周血CD34^+/PMNC值与收缩压呈负相关(r=-0.622,P=0.009),与舒张压、总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、空腹血糖、尿酸水平无相关性。高血压组内皮祖细胞Ang-1和Tie-2 mRNA水平显著低于对照组(P=0.000),与CD34^+/PMNC值分别呈正相关(r=0.561,P=0.005;r=0.473,P=0.009)。结论老年高血压患者的心血管病变可能与Ang-1和Tie-2水平的下调从而影响内皮祖细胞的修复功能有关。

Objective To explore the correlation between circulating CD34^＋ cell level and elderly patients with hypertension and analyze Ang-1/Tie-2 expression in endothelial progenitor cells and its regulatory meaning.Methods Fifty-nine elderly patients with hypertension and 59 healthy people were enrolled and assigned to the hypertension group and the control group,respectively.The mRNA level of Ang-1/Tie-2 was measured by RT-PCR.Flow cytometry was used to detect the CD34^＋ /PMNC value in peripheral blood.The correlations between CD34^＋/PMNC value in peripheral blood and clinical indexes and between Ang-1 and Tie-2 mRNA levels and CD34^＋ /PMNC value were analyzed.Results The CD34^＋ /PMNC value in peripheral blood of the hypertension group was significantly lower than that of the control group（P = 0.000）.The CD34 /PMNC value in peripheral blood of the hypertension group was negatively correlated to systolic pressure（r =- 0.622;P = 0.009）,while was not correlated to diastolic pressure,total cholesterol,triglyceride,high density lipoprotein cholesterol,low density lipoprotein cholesterol levels,fasting blood glucose and uric acid.The Ang-1 and Tie-2mRNA levels in endothelial progenitor cells of the hypertension group were significantly lower than those in the control group（P = 0.000） and were positively correlated to CD34^＋ /PMNC value（r =0.561,P = 0.005;r =0.473,P = 0.009）.Conclusion Cardiovascular lesions in elderly patients with hypertension may be associated with the down-regulation of Ang-1 and Tie-2 levels,which affects repair function of endothelial progenitor cells.