期刊文献+

NADPH氧化酶源性活性氧介导epinodosin诱导HL-60细胞分化及细胞骨架重组 被引量:4

NADPH Oxidase-derived ROS Mediated the Differentiation and Reorganization of Cytoskeletalon Induced by Epinodosin in HL-60 Cells
原文传递
导出
摘要 该文分析研究了对映-贝壳杉烷二萜epinodosin通过调节NADPH氧化酶源活性氧诱导急性早幼粒细胞白血病细胞HL-60向成熟粒细胞分化的特征。结果显示,epinodosin在4.0~8.0μmol/L浓度时抑制HL-60细胞生长,导致其S期阻滞,诱导HL-60细胞形成肾形核和多叶核细胞,使细胞吞噬能力和对硝基四氮唑蓝(nitrotetrazolium blue chloride,NBT)的还原力显著增强、细胞表面抗原CD11b表达上调,表明epinodosin可诱导HL-60细胞向成熟粒细胞分化;epinodosin还诱导HL-60细胞骨架重组,显著促进微管的成束组装以及提高波形纤维含量,上述分化特征与临床诱导分化治疗剂全反式维甲酸(all-trans retinoic acid,ATRA)对HL-60细胞的分化诱导特性极为相似。进一步检测显示,抗氧化剂NAC(N-acetyl-L-cysteine)以及NADPH氧化酶抑制剂夹竹桃麻素(apocynin,APO)和联苯基三价碘(diphenyleneiodonium,DPI)均可抑制epinodosin对HL-60细胞的分化诱导作用,表明epinodosin导致HL-60细胞活性氧浓度的升高与NADPH氧化酶活性关联,提示epinodosin通过调节NADPH氧化酶源性活性氧诱导HL-60向粒细胞分化。 In this paper, NADPH oxidase-derived reactive oxygen species(ROS) mediated the differentiation of HL-60 to mature granulocyte caused by epinodosin were studied. The results showed that the growth of HL-60 was inhibited by epinodosin under the concentration of 4.0~8.0 μmol/L, and it was arrested at S phase. Epinodosin induced HL-60 formed kidney-shaped nuclei and lobulated nuclei, enhanced nitrotetrazolium blue chloride(NBT)-reducing ability, cellular uptake and the expression of CD11 b of HL-60 cells. These indicated that epinodosin could induce the differentiation of human HL-60 cells to granulocyte. And also, epinodosin could induce the reorganization of cytoskeleton in HL-60 cells, improve the assembly of microtubules and vimentin filaments. All the differentiation characteristics mentioned above were similar as the results of clinical differentiation therapy all-trans retinoic acid(ATRA) acted on HL-60 cells. Further detection displayed that the inducement of differentiation by epinodosin was inhibited by N-acetyl-L-cysteine(NAC, an antioxidant) andapocynin/diphenyleneiodonium(APO/DPI, the inhibitors of NADPH oxidase). Namely, the increase of ROS was associated with NADPH oxidase, it revealed NADPH oxidase-derived reactive oxygen species mediated HL-60 cells differentiation caused by epinodosin.
出处 《中国细胞生物学学报》 CAS CSCD 2016年第3期273-284,共12页 Chinese Journal of Cell Biology
基金 国家自然科学基金(批准号:30960464)资助的课题~~
关键词 epinodosin HL-60细胞 分化 活性氧 NADPH氧化酶 细胞骨架重组 epinodosin HL-60 cells differentiation ROS NADPH oxidase cytoskeleton reorganization
  • 相关文献

参考文献29

  • 1Lo-Coco F, Avvisati G, Vignetti M, Thiede C, Orlando SM, Iacobelli S, et al. Retinoic acid and arsenic trioxide for acute promyelocytic leukemia. N Engl J Med 2013; 369(2): 111-21.
  • 2de The H, Le Bras M, Lallemand-Breitenbach V. The cell biology of disease: Acute promyelocytic leukemia, arsenic, and PML bodies. J Cell Bio12012; 198(1): 11-21.
  • 3de The H, Chen Z. Acute promyelocytic leukaemia: Novel insights into the mechanisms of cure. Nat Rev Cancer 2010; 10(11): 775-83.
  • 4Li J, Stouffs M, Serrander L, Banff B, Bettiol E, Charnay Y, et al. The NADPH oxidase NOX4 drives cardiac differentiation: Role in regulating cardiac transcription factors and MAP kinase activation. Mol Biol Cell 2006; 17(9): 3978-88.
  • 5Lawless MW, O'Byrne KJ, Gray SG. Targeting oxidative stress in cancer. Expert Opin Ther Tar 2010; 14(11): 1225-45.
  • 6Ye ZW, Zhang J, Townsend DM, Tew KD. Oxidative stress, redox regulation and diseases of cellular differentiation. Biochimica et BiophysicaActa 2015; 1850(8): 1607-21.
  • 7Guichard C, Pedruzzi E, Fay M, Ben Mkaddem S, Coant N, Daniel F, et al. The Nox/Duox family of ROS-generating NADPH oxidases. Med Sci 2006; 22(11): 953-9.
  • 8George AL, Bangalore-Prakash P, Rajoria S, Suriano R, Shanmugam A, Mittelman A, et al. Endothelial progenitor cell biology in disease and tissue regeneration. J Hematol Oncol 2011; 4(1): 24-31.
  • 9Murray TV, Smymias I, Shah AM, Brewer AC. NADPH oxidase 4 regulates card-iomyocyte differentiation via redox activation of c-Jun protein and the cis-regulation of GATA-4 gene transcription. J Biol Chem 2013; 288(22): 15745-59.
  • 10Chen N, Chen H, Yao Y, Zhang B, Zheng QS. A critical role of redox state in determining HL-60 cell differentiation. Proc Environ Sci 2011; 8: 653-660.

二级参考文献63

共引文献22

同被引文献24

引证文献4

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部