308nm激光联合胡椒碱对表皮黑素细胞中黑素合成及酪氨酸相关蛋白水平的影响

Effects of Combination of 308 nm Excimer Laser with Piperine on the Melanin Synthesis of Epidermal Melanophore and Expression of Tyrosine Related Proteases

目的研究308nm激光联合胡椒碱对表皮黑素细胞中黑素合成及酪氨酸相关蛋白水平的影响,初步探讨不同分组对黑素合成的作用机制。方法培养的表皮黑素细胞分别以308nm激光联合胡椒碱(联合干预组)、308nm激光组、胡椒碱组、阳性对照组及空白对照组作用24h,72h及120h后,测定细胞黑素含量及酪氨酸相关蛋白(TRP-1,TRP-2)活性(激光共聚焦荧光定量分析法)。结果联合干预组、308nm激光组、胡椒碱组、阳性对照组均不同程度促进黑素含量及TRP-1,TRP-2活性的增高。其中以联合干预组促进作用最强。结论 308nm激光联合胡椒碱有明显促进表皮黑素细胞黑素合成作用。

Objective To study the response of the melanin synthesis and the expression of tyrosine related proteases of epidermal melanophore to the combination of 308nm excimer laser with pipeline and discuss the mechanism of melanogenesis in response to different treatment. Methods Epidermal melanophore from the combination intervene group, the 308nm excimer laser group, the piperine group, the positive control group and the control group were incubated for 24h ,72h and 12Oh. Then the melanin content and the expression of TRP-1 and TRP-2 were measured （Quantitative analysis of laser confoeal fluorescence ）. Results The combination intervene group, the 308nm excimer laser group, the pipeline group and the positive control group could promote the cellular melanin content and the activity of TRP-1 and TRP-2 at different degrees. The combination intervene group played the strongest role though. Conclusion 308nm excimer laser combination with piperine can apparently increase the pigmentation of epideImal melanophore.