参苓白术散对有皮肤机械屏障障碍小鼠皮肤屏障功能的影响

Effect of Shen Ling Bai Zhu Powder on Skin Barrier Function of Mice with Mechanical Barrier Disorder

目的通过皮肤机械屏障障碍模型鼠来研究参苓白术散(Shen Ling Bai Shu Power,SLBZP)对皮肤屏障功能的影响。方法取健康小鼠50只,随机分为5组,即正常对照组、模型组、参苓白术散高、中、低剂量组。参苓白术散各组从第1 d起,分别给予参苓白术散(40g/kg、20g/kg、10g/kg)灌胃,其余各组灌胃给予蒸馏水,1次/d,连续13d。除正常对照组外,其余各组从第9天起,以胶带法复制皮肤机械屏障障碍模型,连续5天。观察参苓白术散对皮肤机械屏障障碍模型鼠皮肤组织形态学变化,并测定透皮水丢失(transepidermal water loss,TEWL),表皮神经酰胺含量及表皮厚度。结果造模小鼠皮肤出现相应的组织病理学改变,参苓白术散各组的病理改变均有不同程度的改善;参苓白术散组小鼠背部皮损处经表皮水分丢失量和表皮厚度显著低于模型组(P<0.05,P<0.01);参苓白术散高、中剂量组表皮神经酰胺含量明显高于模型组(P<0.05)。结论参苓白术散可促进皮肤屏障的修复,增加角质层中神经酰胺含量是其可能的机制之一。

Objective To investigate the effect of SLBZP on skin barrier by using a mouse model of skin mechanical barrier disorder. Methods Fifty healthy mice, were randomly divided into 5 groups ： normal control group, model group, and high, medium and low dose SLBZP groups. SLBZP groups were orally administered SLBZP （40g, 20g, 10g/kg）, the other groups were given distilled water, 1 times/d, continuously for 13 days. In addition to the normal control group, on day 9 skin of other groups, were damaged using adhesive tape, continuously for 5 day. Then skin pathology were observed and Transepidermal Water Loss （ TEWL）, concentration of epidermal ceramides, and thickness were examined. Results Pathological changes were much obvious in the adhesive tape damaged group. Pathological changes in each SLBZP group showed different degrees of improvement. TEWL and thickness of the epidermis in SLBZP groups were significantly lower than that of control groups （P 〈 0.05, P 〈 0. 01 ）. Concentration of epidermal ceramides in SLBZP groups （40g, 20g/kg） were higher than those in control group （P 〈 0.05）. Conclusion SLBZP promotes the repair of skin mechanical barrier. Increase in the content of the stratum corneum ceramide is one of possible mechanisms.