巢式聚合酶链反应检测血清中HBV-DNA对治疗乙肝的临床意义

Clinical Significance of the Serum HBV-DNA was Detected by Nested Polymerase Chain Reaction in the Treatment of Hepatitis B

目的:探讨巢式聚合酶链反应(n PCR)检测血清中乙型肝炎病毒(HBV)-DNA对治疗乙型肝炎的临床意义。方法:146名HBV感染者用ELISA检测HBVM作为参照,再分别用FQ-PCR和n PCR检测参与研究的所有HBV患者HBV-DNA,并判断HBV的复制和活动情况。对照组另外选择49名健康者作为对照。结果:模式1和模式4中的HBV基因的阳性率都很高,模式1的阳性率与n PCR和FQ-PCR检测HBV基因的阳性率差距显著(P<0.05)。模式2阳性率与n PCR和FQ-PCR检测HBV基因的阳性率差距不显著(P>0.05)。n PCR法和FQ-PCR法的结果对比可见,n PCR的阳性率显著高于FQ-PCR(P<0.05)。n PCR的阳性率要比ELISA的阳性率高很多(P<0.05)。ELISA阳性率比对照组高很多(P<0.05)。结论:采用n PCR检测技术诊断HBV疾病的检出率高、特异性强,对HBV疾病的诊断具有很高的实用价值。

[ABSTRACT]Objective:To investigate the clinical significance of nested polymerase chain reaction (nPCR) was detected in the sera of hepatitis B virus (HBV) DNA on the treatment of chronic hepatitis B. Methods: 146 patients with HBV were detected by ELISA as a reference, and then FQ-PCR and nPCR were used to detect the HBV-DNA of all HBV patients involved in the study, and to determine the replication and activity of HBV. In control group, the other 49 healthy persons were selected as control group. Results:The positive rate of HBV gene in model 1 and model 4 was very high, the positive rate of model 1 was significant (P<0.05), and the positive rate of HBV gene was detected by nPCR and FQ-PCR. The positive rate of model 2 was not significant (P>0.05), and the positive rate of HBV and FQ-PCR was not significant (nPCR). Compared with the results of nPCR method and FQ-PCR method, the positive rate of nPCR was significantly higher than that of FQ-PCR (P<0.05). The positive rate of nPCR was much higher than that of ELISA (P<0.05). The positive rate of ELISA was much higher than that of control group (P<0.05). Conclusion: The detection rate of HBV is high, the specificity is strong, and it has a high practical value for the diagnosis of HBV disease by using nPCR detection technology.