摘要
以黑曲霉A-15形成的菌丝球作为载体对酿酒酵母C-15进行固定化,研究各种因素对黑曲霉A-15菌丝球吸附率的影响。结果表明:在40℃、80 r/min、菌种个数混合比例为1∶10时,菌丝球对酵母的吸附可达到66.9%;以菊粉为底物,利用混合菌丝球进行同步糖化与发酵,30℃发酵48 h,发酵醪酒精体积分数达到12.8%,并且混合菌丝球可以耐受19%的酒精。
Mycelial pellets formed by Aspergillus niger A?15 were used to immobilize the alcohol producing yeast Saccharomyces cerevisiae C?15. The operated factors, such as agitation speed, temperature and mixed proportion of strains were studied. The optimal adsorption 66?9% was obtained when speed was 80 r/min, temperature was 40℃ and mixed proportion was 1∶10. With Jerusalem artichoke flour as substrate,12?8%( V/V) of ethanol was produced within 48 h of fermentation by simultaneous saccharification and fermentation using mycelial pellets at 30℃. And mycelial pellets could tolerate 19%(volume fraction) alcohol.
出处
《生物加工过程》
CAS
2016年第2期12-16,共5页
Chinese Journal of Bioprocess Engineering
基金
山东大学微生物技术国家重点实验室开放基金项目(M2012-14)
济南市引进海内外高层次人才创业项目(2011044)
山东省住房城乡建设科学技术项目计划(KY007)
关键词
菌丝球
载体发酵
乙醇
同步糖化发酵
mycelial pellet
carrier fermentation
alcohol
simultaneous saccharification and fermentation
