M.hydrocarbonoclasticus NY4中N_2OR的酶活测定及环境因素对酶活力的影响

Determination and characterization of nitrous oxide reductase in Mourinobacter hydrocarbonoclasticus NY4

氧化亚氮（N2O）是一种主要的温室气体,其生物学减量控制已经成为研究热点.在自然环境中,氧化亚氮还原酶（N2OR）是唯一能将N2O 还原成N2 的酶.以1 株能在高盐条件下进行厌氧及好氧反硝化作用的海杆菌Marinobacter hydrocarbonoclasticus NY4 为研究对象,建立了一种稳定的N2OR 酶活检测方法,考察了pH、盐质量分数以及碳氮比（C/ N）等环境因素对N2OR 酶活力的影响.结果发现：当pH 为7 时、盐质量分数为6%～8%、C/ N 为8 时,N2OR 呈现出了最高的酶活.本实验结果丰富了对N2OR 酶学特征的了解,为N2O 生物减量控制提供了部分参考.

Nitrous oxide ( N2 O) is a major greenhouse gas,and its biological reduction has become a hot topic. Nitrous oxide reductase ( N2 OR ) is the only enzyme that can reduce N2 O to N2 in natural environments. Marinobacter hydrocarbonoclasticus NY4,a halophilic bacterium with the ability of anaerobic and aerobic denitrification,was used in this study?We established a stable method to determine the activity of N2 OR,and studied the influence of key environment factors on the activity of N2 OR including pH value, salt concentration and carbon/nitrogen ratio ( C/N) . When pH was at 7,the salinity was 6% and C/N was 8,strain NY?4 exhibited the highest N2 OR activity. The experimental data enriched the knowledge of the characteristics of N2 OR,and provided some references for the biological reduction of N2 O.