C-erBb-2,VEGF和MMP-9在大肠癌中的表达及其相关意义

Expression of C-erBb-2,VEGF,and MMP-9 in Laryngeal Carcinoma and Its Clinical Significance

目的探讨大肠癌中血管内皮生长因子(VEGF)、C-er Bb-2和基质金属蛋白酶(MMP-9)的表达及其与临床病理特征的关系。方法选择大肠癌组织60例和远癌大肠黏膜20例;采用免疫组织化学方法检测VEGF,C-er Bb-2和MMP-9的阳性表达,并对其表达与大肠癌的临床病理特征的关系以及三者间的相关性进行分析。结果在大肠癌组织中,C-erb B-2、VEGF和MMP-9的阳性表达率分别为50%、60%、60%,明显高于远癌肠组织(P<0.01)。大肠癌组织Cer Bb-2、VEGF和MMP-9阳性表达与年龄、性别和分化程度无关(P>0.05),而与肿瘤侵袭深度、淋巴结转移和病理分期密切相关(P<0.05)。C-er Bb-2,VEGF和MMP-9在大肠癌中的表达均呈正相关;VEGF和C-erb B-2间的等级相关系数为0.461;MMP-9和VEGF间的等级相关系数为0.483,C-erb B-2和MMP-9间的等级相关系数为0.427。结论 C-er Bb-2,VEGF和MMP-9的表达与大肠癌组织浸润、淋巴结转移和临床分期有关;提示可作为判定大肠癌发生、发展以及预后的指标。

Objective To investigate the expression of C-erBb-2, vascular endothelial growth factor（ VEGF）, and matrix metalloproteinase-9 （ MMP-9 ） in laryngeal carcinoma and its correlation with clinical pathological factors. Methods 60 laryngeal carcinoma tissues and 20 normal tissues of large intestine were chosen. Immunohistochemistry method was employed to detect the expression of C-erBb-2, VEGF, and MMP-9 in all tissues. The relationship between expression of C-erbB-2, VEGF, and MMP-9 with clinicopathological factors of laryngeal carcinoma tissues was analyzed, and at the same time correlation among C-erbB-2, VEGF, and MMP-9 was also analyzed. Results The positive rates of C-erbB-2, VEGF, and MMP-9 in laryngeal carcinoma tissues were 50% ,60% , and 60% respectively, which were obviously higher than those of normal tissues of large intestine （P 〈 0.01 ）. There was statistical correlation between expression of C-erBb-2, VEGF, and MMP-9 in [argengeal carcinoma and tumor invasion, metastasis, and pathological grade （ P 〈 0.01, P 〈 0.05 ）, but was not related with age, sex, and differentiation （ P 〉 0.05 ）. Expression of C-erBb-2, VEGF, and MMP-9 were positive correlated in laryngeal carcinoma tissues. Coefficient of rank correlation between VEGF and C-erbB-2 was 0. 461 ,MMP-9 and VEGF was 0. 483,C-erbB-2 and MMP-9 was 0. 427. Conclusion Expression of C-erBb-2, VEGF, and MMP-9 in laryngeal carcinoma tissues are significantly related to pathological grade, clinical stage, and metastasis, it can be marker evaluating occurrence, development, and prognosis of laryngeal carcinoma.