摘要
目的研究云南省目前艾滋病病毒1型(HIV-1)流行毒株的亚型分布特点。方法收集2012-2014年云南省14个地市731例在定点医院随访的HIV感染者或艾滋病患者的血样及流行病学调查信息,采用反转录聚合酶链反应(RT-PCR)扩增HIV-1的3′半分子约4.5kb(包含env基因全长),序列校对后用Genotyping、MEGA6.06和BLAST工具软件确定毒株亚型,分析云南省HIV-1的地区和人群分布特征。结果共获得731例样本的224条半分子序列,各亚型HIV-1毒株及其所占的比例分别为CRF08_BC 58.0%,CRF01_AE 18.3%,CRF07_BC11.6%,未知重组8.0%,B(B′)亚型2.7%和C亚型1.3%。在德宏州和西双版纳主要以CRF01_AE为主,其余地州以CRF08_BC为主,在临沧、红河、玉溪、曲靖CRF08_BC占绝对优势(>70%)。以上6种亚型的人口学特征(性别、年龄、婚姻状况、民族)及感染途径分布差异均无统计学意义(P>0.05),B(B′)或C与CRF01_AE、B与C两种未知型重组在传播途径上的分布差异亦无统计学意义。结论运用3′半分子来确定病毒亚型,和前期2008-2009年的研究比较,云南省的HIV-1毒株在地域、人口学、传播途径等特征的分布上较之前有变化,仍有新型重组毒株出现,应密切监测流行趋势变化。
Objective To investigate the genetic subtypes and the characteristics and distribution of HIV-1strains prevalent in Yunnan.Methods 731HIV/AIDS plasma specimens and epidemiological information were collected in 2012-2014 from 14prefectures of Yunnan.Viral RNA were extracted and then amplified using RT-PCR,about 4.5kb 3'halves fragment obtained and directly sequenced,sequences collated and then HIV-1subtypes were determined by Genotyping,MEGA 6.06 and BLAST analysis,described regional and population distribution of HIV-1.Results Of the 731 plasma specimens,a total number of 224 genomic sequences were successfully amplified and sequenced.After genotype identified,the subtypes of HIV-l strains were prevalent with the order of constituent ratio CRF08_BC(58.0%),CRF01_AE(18.3%),CRF07_BC(11.6%),unknown recombinant forms(8.0%),B(B′)(2.7%)and C(1.3%).HIV-1had features of geographical distribution.CRF01_AE was dominant in the areas of Dehong and Xishuangbanna,and CRF08_BC was prevailing in Lincang,Honghe,Yuxi,Qujing,and other areas by more than 70.0%.However,the subtypes distributions of HIV-1in demographic characteristics showed no statistical significance(P〉0.05).There was no statistical difference between the distributions of the two unknown recombinant forms B(B′)C and CRF01_AE with subtype B or C in modes of infection.Conclusion In the case of HIV-1molecule,3'halves are used for genotyping.Compared with previous researches conducted in 2008-2009,subtypes of HIV-1strains prevalent in Yunnan show some differences in the distributions of geography,demography and transmission routes.More new recombinants have emerged and the epidemiological situation should be monitored closely.
出处
《中国艾滋病性病》
CAS
北大核心
2016年第3期145-149,共5页
Chinese Journal of Aids & STD
基金
云南省科技厅应用基础研究计划自筹项目(2013FZ217)
国家"十二五"传染病科技重大专项(2012ZX10001-002)~~
关键词
艾滋病病毒-1
流行毒株
亚型
分布特征
HIV type 1
prevalent strains
Genetic subtype
Distribution characteristics