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1,25-(OH)_2维生素D3对再生障碍性贫血患者T细胞亚群的影响 被引量:1

Effects of 1,25-(OH)_2 D3 on T cell subsets in patients with aplastic anemia
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摘要 目的:研究再生障碍性贫血(AA)患者外周血中的T细胞亚群变化与血清中1,25-(OH)_2维生素D3水平的关系。方法:利用流式细胞仪检测15例AA患者和25例健康对照者外周血T细胞亚群的变化,并用ELISA法检测AA患者和健康对照者血清中1,25-(OH)_2维生素D3的水平。结果:AA患者血清中1,25-(OH)_2维生素D3水平明显低于健康对照者[(35.5±11.8)pmol/L∶(51.8±12.6)pmol/L,P<0.05]。与健康对照者比较,AA患者外周血中CD4在淋巴细胞中比率无明显变化[(31.8±4.7)%∶(33.6±4.2)%,P=0.217],但CD8在淋巴细胞中比率显著升高[(31.5±5.2)%∶(24.8±3.8)%,P<0.01],故CD4/CD8比值降低(P<0.01);其中在CD4^+细胞中,TH1/TH2比值较健康对照者明显升高[(4.09±1.05)∶(3.12±0.89),P=0.003]。结论:AA患者外周血清中1,25-(OH)_2维生素D3水平异常降低,可能影响AA患者CD4^+T细胞的分化。 Objective:To study the effect of 1,25-(OH)_2 D3 on T cell subsets in patients with aplastic anemia(AA).Method:Fresh peripheral blood samples of 15 patients with AA and 25 normal controls were analyzed for the level of 1,25-(OH)_2 D3 with ELISA.The T cell subsets were detected by flow cytometry.Result:The median level of serum 1,25-(OH)_2 D3 of AA patients was lower than that of normal controls[(35.5±11.8)pmol/L vs.(51.8±12.6)pmol/L,P〈0.05].There was no significant difference of the median number of CD4~+ cells between AA patients and normal controls[(31.8±4.7)% vs.(33.6±4.2)%,P=0.217].The median number of CD8~+ cells in AA patients was higher than that in normal controls[(31.5±5.2)% vs.(24.8±3.8)%,P〈0.01].In CD4~+ cells,the ratio of TH1/TH2 of AA patients was higher than that of normal controls [(4.09±1.05)vs.(3.12±0.89),P=0.003].Conclusion:The level of serum 1,25-(OH)_2 D3 is decreased in AA patients,which may affect the differentiation of CD4~+ T cell in AA patients.
出处 《临床血液学杂志》 CAS 2016年第2期207-208,212,共3页 Journal of Clinical Hematology
关键词 1 25-(OH)2维生素D3 再生障碍性贫血 T细胞亚群 1 25-(OH)2 D3 aplastic anemia T cell subsets
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