摘要
Human induced pluripotent stem cells （hiPSCs） can self-renew indefinitely and have the potential to differentiate into all cell types in the human body, thus hold great promise in regen- erative medicine, drug screening and developmental biology studies. However, integrating retroviral （Takahashi et al., 2007） or lentiviral （Hockemeyer et al., 2008） gene delivery systems and the exogenous oncogene c-Myc （Takahashi et al., 2007; Hockemeyer et al., 2008） have been typically used in the reprogramming process, which could increase risks of insertional mutations or epigenetic abnormalities and conse- quently pose biosecurity concerns for basic research and clinical applications of hiPSCs.
Human induced pluripotent stem cells （hiPSCs） can self-renew indefinitely and have the potential to differentiate into all cell types in the human body, thus hold great promise in regen- erative medicine, drug screening and developmental biology studies. However, integrating retroviral （Takahashi et al., 2007） or lentiviral （Hockemeyer et al., 2008） gene delivery systems and the exogenous oncogene c-Myc （Takahashi et al., 2007; Hockemeyer et al., 2008） have been typically used in the reprogramming process, which could increase risks of insertional mutations or epigenetic abnormalities and conse- quently pose biosecurity concerns for basic research and clinical applications of hiPSCs.
基金
supported by grants from the National Basic Research Program of China (No. 2013CB967102)
the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA01030506)
the International S&T Cooperation Program of China (No. 2013DFG30680)
