中国新疆小反刍兽疫病毒F基因序列分析

Sequence Analysis of F Gene of Peste Des Petits Ruminants in Xinjiang

为分析中国新疆小反刍兽疫病毒（PPRV）毒株分子演变特点和疫情传播路线,从GenBank数据库下载所有国家全部的PPRVF基因全序列,应用DNAStar软件,结合疫情毒株时空分布进行序列分析。结果显示,中国新疆PPRV株F基因全序列,与2014年小反刍兽疫情中河南和北京毒株核苷酸的同源性最高,为99.8%～99.9%,与中国西藏3个毒株核苷酸的同源性为97.7%,与中国采用的疫苗株核苷酸同源性为92.9%,核苷酸同源性最低的是肯尼亚毒株,与国外毒株核苷酸同源性最高的是印度毒株。F基因系统进化关系分析显示,中国新疆PPRV株属于基因Ⅳ系。F蛋白氨基酸同源性结果显示,除与科特迪瓦1989年毒株ICV89氨基酸同源性最低外,与其他氨基酸同源性比对结果均与核苷酸的比对结果一致。中国新疆PPRV株存在一定程度的变异,可能是由周边国家传入。

In order to analyze the molecular evolution characteristic of peste des petits ruminants virus（PPRV）and the epidemic disseminated route in Xinjiang.We downloaded the complete sequences of F gene of PPRV from GenBank of all the countries in the world.We used DNAStar software and combined it with spatial and temporal distribution of strains from NCBI to analyze its sequence.The results showed that the nucleotide homology of Xinjiang strain was the highest with the epidemic strainsof other places in China,the nucleotide homology with Tibet strain which was epidemic before,and with Chinese vaccine strain used were 97.7% and 92.9% respectively.The nucleotide homology with Kenya strain was the lowest,with India strain was the highest among foreign strains.The phylogenetic analysis of Fgene showed that Xinjiang strain belongs to the gene seriesⅣ.The amino acid homology of F protein was consistent nearly with the nucleotide homology,but was the lowest with ICV89 strain from Ivory Coast.There are certain variation in the PPRV strain,it may be introduced into the neighboring countries.