摘要
修饰核苷是RNA的代谢产物,目前认为是一种广谱的潜在肿瘤标志物(tumor marker,TM),在肿瘤患者尿液中排放量较正常人显著升高。液相色谱-质谱联用(liquid chromatography-mass spectrometry,LC-MS)技术结合了色谱的高分离能力和质谱的高选择性、高灵敏度及能够提供相对分子质量与结构信息的优点,已逐渐成为修饰核苷分析的的主要技术平台之一。本文综述了近年来LC-MS技术在尿液修饰核苷分析方面的现状与进展,并对其发展前景进行展望。
Modified nucleosides are the RNA metabolites, and are well known as potential marker in various types of cancer. They are mainly detected in urine wherein thier concertrations were found elevated in cancer patients. Liquid Chromatography-Mass Spectrometry （LC-MS） technique has been widely used in the field of modified nucleosides analysis, which can not only reach the point of high separating capacity of chromatography but also be provided with high selectivity, sensitivity, and relative molecular mass and structure information of mass spectrum. This review deals with the current status and future trends in the analysis of urinary modified nucleosides as tumor markers by LC-MS technique.
出处
《中国生化药物杂志》
CAS
2016年第2期182-185,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
浙江省医药卫生科技计划(2016KYA034)
关键词
修饰核苷
肿瘤标志物
液相色谱-质谱联用
综述
modified nucleosides
tumor marker
liquid chromatography-mass spectrometry （LC-MS）
review
