HIF-2、Twist在胰腺癌组织中的表达及其临床意义

Expressions and significance of HIF-2 and Twist in pancreatic cancer

目的探讨缺氧诱导因子2(HIF-2)和Twist在胰腺癌组织中的表达及其临床意义。方法采用免疫组化SP法检测56例胰腺癌及其癌旁组织中HIF-2和Twist的表达,分析其与临床病理指标的关系。结果 HIF-2阳性染色主要定位于细胞质,HIF-2在胰腺癌组织中阳性表达率高于癌旁组织(76.8%vs.28.6%)(P<0.01)。Twist阳性染色定位于细胞质或细胞核,Twist在胰腺癌组织阳性表达率高于癌旁组织(69.6%vs.21.4%)(P<0.01)。HIF-2阳性表达与肿瘤大小、临床分期、淋巴结转移及血管侵犯密切相关(P<0.01或P<0.05)。Twist阳性表达与淋巴结转移及血管侵犯密切相关(P<0.01)。HIF-2与Twist在胰腺癌组织中的表达呈正相关(r=0.65,P<0.01)。结论 HIF-2和Twist在胰腺癌组织中高表达;HIF-2和Twist的表达与肿瘤侵袭和转移密切相关,可能成为潜在的抗肿瘤治疗靶点。

Objective To explore the expressions and significance of hypoxia inducing factor（HIF）-2 and Twist in pancreatic cancer.Methods The expressions of HIF-2 and Twist in 56 pairs of pancreatic cancer tissues and tumor-adjacent mucosa were detected by immunohistochemistry SP method.The relationship between the expressions and clinicopathologic indicators was analyzed.Results HIF-2 positive staining mainly located in cytoplasm.The positive expression rate of HIF-2 was higher in pancreatic cancer than that in tumor-adjacent mucosa（76.8% vs.28.6%）（P〈0.01）.Twist positive staining located in cytoplasm or nucleus.The positive expression rate of Twist was higher in pancreatic cancer than that in tumor-adjacent mucosa（69.6% vs.21.4%）（P〈0.01）.The expression of HIF-2 was closely related to tumor size,clinical staging,lymph node metastasis and vascular invasion（P〈0.01 or P〈0.05）.The expression of Twist was closely related to tumor invasion and metastasis（P〈0.01）.HIF-2 expression was positively correlated to Twist expression in pancreatic cancer tissues（r=0.65,P〈0.01）.Conclusion HIF-2 and Twist are highly expressed in pancreatic cancer,which are closely associated with tumor invasion and metastasis and could be the potential targets for antitumor therapy.