DCLK1在结直肠癌、结直肠腺瘤中的表达及临床研究

Expression of doublecortin-like kinase 1 in colorectal cancers and colorectal adenomas and its clinical significance

目的 检测Doublecortin样激酶-1（DCLK1）在结直肠癌、结直肠腺瘤患者组织中的表达,分析DCLK1在结直肠肿瘤发生发展过程中的作用,探索糖尿病史对DCLK1表达及肿瘤患者预后的影响.方法 采用免疫组织化学法（SP法）检测70例结直肠癌组织、70例正常癌旁组织及35例管状绒毛状腺瘤中DCLK1的免疫表达水平.结果 （1）DCLK1在结直肠癌的高表达率为41.4％（29/70）,在管状绒毛状腺瘤高表达率为45.7％ （16/35）,均明显高于正常癌旁结肠组织中高表达率的4.3％（3/70）（P＜0.01）.（2）DCLK1的高表达与患者病理学分级（P＜0.01）、浸润深度（P＜0.05）、淋巴结转移（P＜0.05）、脉管侵犯（P＜0.05）、远处转移（P＜0.01）有关.（3）糖尿病史与DCLK1的表达之间及肿瘤患者预后无明显相关性.结论 DCLK1在结直肠肿瘤中存在异常高表达,对结直肠肿瘤的早期临床诊断及预后有一定临床意义;糖尿病史与结直肠癌DCLK1的表达及生存时间无明显关联.

Objective To investigate the expression of doublecortin-like kinase 1 （DCLK1） in colorectal cancer and colorectal adenomas,and it＇s role in the development and progression of colorectal carcinoma,and explore the relationship of the DCLK1 expression and prognosis in colorectal cancer patients with diabetes medical history.Methods Expression of DCLK1 in 70 cases of colorectal cancers,70 cases of para-carcinoma tissues,and 35 cases of tubulovillous adenomas were detected by immunohistochemical method.Results （1） The DCLK1 high expression rate in colorectal cancers was 41.4% （29/70）,and 45.7% （16/35） in tubular villous adenomas,both significantly higher than the para-carcinoma tissues （4.3%;3/70） （P 〈0.01）.（2） DCLK1 high positive expression had statistical significance with pathological grading （P 〈 0.01）,infiltration depth （P 〈 0.05）,lymph node metastasis （P〈 0.05）,vascular invasion （P 〈0.05）,and distant metastasis （P 〈0.01）.（3） There was no obvious correlation between the Diabetes medical history,expression of DCLK1,and prognosis of patients.Conclusions DCLK1 has high positive expression in colorectal carcinomas,and may have certain significance for the early clinical diagnosis and prognostic judgment of colorectal cancers.The expression of DCLK1 and prognosis of colorectal cancers has no obvious correlation with diabetes medical history.