牛粪堆肥中厌氧氨氧化菌分子生物学检测

Molecular detection of anaerobic ammonium oxidation bacteria in cow manure composting

研究利用PCR-DGGE方法对堆肥中厌氧氨氧化菌16S r RNA基因特异扩增,检测堆肥厌氧氨氧化菌种属及多样性水平。结果表明,牛粪堆肥中厌氧氨氧化菌群组成与海洋环境不同。海洋环境中Candidatus Scalindua属厌氧氨氧化菌为优势菌群,而牛粪堆肥中厌氧氨氧化菌中优势菌属为Candidatus Brocadia、Candidatus Kuenenia和Candidatus Scalindua,多样性相对较高。可为堆肥发酵过程中厌氧氨氧化过程提供分子生物学依据。

In this study, the species and diversity of anammox bacteria 16S rRNA gene in composting process were detected by PCR-DGGE technology. The results showed that anammox bacterial genera in cow manure composting were different from the marine environment. CandJdatus Scalindua was dominat anammox bacteria in marine environment, while Candidatus 8rocadia, Candidatus Kuenenia and Candidatus Scalindua were dominant anammox bacteria in cow manure compost, and anammox bacteria diversity was relatively high. This study provided the molecular evidence for the existence of anammox during the composting process.