昆明地区汉族维生素D受体基因多态性与2型糖尿病的相关性

The Correlation between Vitamin D Receptor Gene Polymorphism and Type 2 Diabetes Mellitus of the Han Nationality in Kunming Region

目的探讨昆明地区汉族人群维生素D受体基因多态性与2型糖尿病的相关性.方法选取昆明地区汉族2型糖尿病患者（DM组）90例及健康对照（NC组）36例.运用PCR-FRLP技术测定维生素D受体Apa I基因多态性,比较各组基因型及等位基因频率及不同基因型间相关临床资料.结果 （1）2型糖尿病组与正常组间维生素D受体Apal基因存在多态性,2型糖尿病组中C/A基因型分布频率明显高于正常对照组（χ^2=53.432,P〈0.001）;（2）2型糖尿病组的年龄、FPG、LDL-C高于正常对照组,25-OHD3、Ca低于正常对照组（P〈0.05）;糖尿病组A/A基因型Scr显著高于其他组别,差异有统计学意义（P〈0.05）;3组基因型间UA均存在统计学差异（P〈0.05）,且C/A基因型最高;（3）二分类Logistic回归分析结果提示Apa I C/A基因型（OR=1.36,95%CI：0.54-1.71）、年龄（OR=1.09,95%CI：1.03-1.18）为2型糖尿病的危险因素,而25-OHD3（OR=0.84,95%CI：0.73～0.93）可能是T2DM的发生的保护因素.结论 （1）昆明地中VDR Apa I位点C/A基因型与T2DM的发生存在相关性;（3）25-OHD3是T2DM的发生的保护因素,增龄是2型糖尿病的危险因素.

Objective To explore the correlation between vitamin D receptor（ VDR） gene polymorphism and type 2 diabetes mellitus of the Han nationality in Kunming region. Methods One- hundred- twenty- six Kunming individuals（ 90 T2 DMs and 36 controls） were enrolled in this randomized controlled trial. rs7975232 single nucleotide polymorphism（ SNPs） of the VDR was tested by using Polymerase chain reaction of restriction fragment length polymorphism（ PCR- RFLP）, the genotype and its frequencies were compared between the two groups.Results（ 1） There were statistically significant association in three kinds of genotype of Apa I of VDR gene polymorphism between two groups（ P〈0.001）. The C/A genotype was more common in T2 DM patients（ 62.2% vs38.9%）.（ 2） Statistic analysis software showed that the frequency of C/A genotype of Apa I was higher than that in the control group（ OR=1.36, 95%CI： 0.54- 1.71）. Whats more, age in T2 DM group were also higher than that in the control group（ OR= 1.09, 95% CI： 1.03- 1.18）. 25OHD3 in T2DM group was lower than that in the control group（ OR=0.84, 95%CI：0.73- 0.93）, suggesting that C/A genotype and age are risk factors of T2 DM. Meanwhile25OHD3 may be a preventive factor of T2 DM.Conclusions（ 1） There is a significant correlation between C/A genotype of rs7875232 and the prevalence of T2 DM of the Han nationality in Kunming region.（ 2） 25OHD3 is aprotective factor of T2 DM and the aging is a risk factor of T2 DM.