不饱和糖功能单体接枝聚氨酯膜的制备及其血液相容性

Preparation and Hemocompatibility of Polyurethane Films Grafted with an Unsaturated Sugar-Based Functional Monomer

通过葡萄糖、丙烯酸羟乙酯和丁二胺反应,制备了含不饱和双键的糖基功能单体。采用傅里叶红外光谱和核磁共振氢谱对合成的产物进行结构表征确定。采用紫外光引发接枝聚合技术,将制备的不饱和糖单体接枝聚合到聚氨酯膜的表面,以衰减全反射模式下傅里叶红外光谱对表面接枝反应进行了确认。通过静态水接触角实验和血小板黏附实验,分别对改性聚氨酯膜表面的亲水性和血液相容性进行了研究,结果表明,改性聚氨酯膜表面的接触角从86°降低到45°,血小板的粘附量由14.36×103cells/mm2减少到2.57×103cells/mm2,亲水性明显增强,血液相容性显著改善。

A sugar-based functional monomer containing double bond was synthesized by the reaction of Dglucose,hydroxylethyl acrylate and 1,4-butanediamine. The as-prepared compound was confirmed by Fourier transform infrared spectroscopy（ FTIR） and proton nuclear magnetic resonance spectroscopy（1H NMR）. The unsaturated sugar monomer was grafted onto the surface of polyurethane（ PU） films by UV-induced grafting polymerization. The surface grafting polymerization was confirmed by FTIR in the attenuated total reflection mode（ ATR-FTIR）. Water contact angle measurement and platelet adhesion were used to study the hydrophilicity and hemocompatibility of the modified PU films,respectively. It is found that the contact angle of modified PU films decreases from 86° to 45°,and the amount of platelet adhesion is reduced from 14. 36 ×10^3cells / mm^2 to 2. 57 × 10^3 cells / mm^2. The hydrophilicity of the modified PU films is obviously enhanced and a more hemocompatible interface can be obtained between the film and the biomolecules.