正交试验优化清解雾化液的提取工艺

Optimization of the Extraction Technology of Qingjie Atomized Liquid by Orthogonal Test

目的:优化清解雾化液的提取工艺。方法:以金丝桃苷含量为考察指标,以加水量、提取时间、提取次数为考察因素,采用正交试验优化清解雾化液的提取工艺并进行验证。结果:最优提取工艺为药材加12倍量水提取2次,每次1 h;3次工艺验证试验中金丝桃苷平均含量为4.129μg/g(RSD=1.81%,n=3)。结论:优化工艺稳定、可行,可为清解雾化液的工艺及质量标准的建立提供依据。

OBJECTIVE：To optimize the extraction technology of Qingjie atomized liquid. METHODS：The extraction technology of Qingjie atomized liquid was optimized by orthogonal test with hyperoside content as index,using the amount of added water,extraction time and extraction times as factors,and the verification test was conducted. RESULTS：The optimal extraction technology was as follows as 12-folds water,extracting for 2 times and 1 hour each time. The content of hyperoside was 4.129 μg/g in3 validation tests（RSD=1.81%,n=3）. CONCLUSIONS：The optimal extraction technology is stable and practical,and can provide reference for the formulation of technology and quality standard for Qingjie atomized liquid.