复方龙芩草微乳提取液体外抗H1N1流感病毒的作用研究

Study on the anti-influenza virus H1N1 activity of microemulsion extract of cmpound Longqincao in vitro

[目的]考察生物相容性微乳及复方龙芩草微乳提取液体外抗H1N1流感病毒的效果。[方法]运用四甲基偶氮唑蓝比色法(MTT)检测微乳和复方龙芩草微乳提取液对A549细胞的最大无毒浓度(TC_0)及半数抑制浓度(TC_(50)),采用细胞病变效应(CPE)法表示药物对病毒感染细胞的作用,MTT法检测细胞病变抑制率(ER)。采用Probit回归法计算受试药物在体外抗H1N1流感病毒的半数抑制浓度(IC_(50))和治疗指数(TI)。[结果]微乳在一定程度上抑制H1N1流感病毒对细胞的感染作用,IC_(50)为769.178 mg/L(以含微乳量计),TI为9.078;复方龙芩草微乳提取液具有显著的抗H1N1流感病毒的效果,当浓度为1 250 mg/L(以含微乳量计)时,抗病毒效率达到91.37%,IC_(50)为314.808 mg/L,TI为20.889。[结论]复方龙芩草微乳提取液体外抗H1N1流感病毒的效果显著,微乳具有一定的抗流感病毒的效果,在复方龙芩草微乳提取液中或许起到辅助作用。

[Objective] To study the antiviral effects of biocompatible microemulsion and microemulsion extract of cmpound Longqincao on H1N1 influenza virus in vitro. [Methods] The maximum non-toxic concentration（TC0） and median toxic concentration（TC50） of biocompatible microemulsion and microemulsion extract of cmpound Longqincao on A549 cells were detected by using methyl thiazolyl tetrazolium（MTT）. The influences of these drugs on virus-infected cells were observed by cytopathic effect（CPE）, the cytopathic inhibition rate（ER） was detected by using MTT. The median inhibitory concentration（IC50） and therapeutic index（TI） of these two drugs on viral infection were calculated by the Probit regression. [Results] Microemulsion had inhibitory effect on the pathogenic effect of influenza virus, IC50 was 769.178 mg/L（Calculated with microemulsion） and TI was 9.078. When the concentration of microemulsion extract of cmpound Longqincao was 1 250 mg/L, The antiviral rate was 91.37%. IC50 was 314.808 mg/L（Calculated with microemulsion）and TI was 20.889. It had a significant effect on protecting A549 cells from intrusion. [Conclusion] The microemulsion extract of cmpound Longqincao played a good role in anti-H1N1 influenza virus, the microemulsion also had antiviral effect, and maybe played a supporting role to a certain extent.