摘要
目的观察去除颈动脉窦和损伤下丘脑视前区(POA)对外周给精氨酸加压素(AVP)引起大鼠低温的影响。方法实验用成年雄性SD大鼠,去除颈动脉窦或用卡因酸损伤POA。在22℃环境中,用无线遥控测温技术连续记录大鼠体温和活动的变化,10∶00 AM给大鼠腹腔注射AVP(10μg/kg)或生理盐水(等容量)。结果去除颈动脉窦能明显减弱腹腔注射AVP引起低温反应(-1.26±0.32℃),与假手术大鼠降温效应(-1.95±0.22℃)比较差异有统计学意义(P<0.01)。损伤POA大鼠,腹腔注射AVP引起的低温反应幅度(-0.8±0.17℃)明显小于假手术大鼠(-1.56±0.22℃,P<0.01)。结论去除颈动脉窦和损伤POA均能够减弱腹腔注射AVP引起的低温反应,提示颈动脉窦压力感受器和POA参与外周给AVP引起的低温反应。
Objective To observe the effect of sinoaortic denervation （SAD） and preoptic area （POA） lesioned on arginine vasopressin（AVP）-induced hypothermia in rats. Methods Male Sprague-Dawley rats were submitted to sinoaortic denervation or kainic acid lesioning of the POA. Core temperature and motor activity were monitored by telemetry at an ambient temperature of 22℃. Rats were dosed intraperitoneally at 10∶ 00 with AVP （10μg/kg） or saline （the same volume）. Results Sinoaortic denervation （SAD） significantly prevented hypothermia induced by intraperitoneal injection of AVP when compared to sham rats （-1.260.32℃ AVP-SAD vs -1.950.22℃ AVP-sham, P〈0.01）. POA lesioned rats treated with AVP had a attenuated decrease in core temperature to sham rats （-0.80.17℃ AVP-POA lesioned vs -1.560.22℃ AVP-sham, P〈0.01）. Conclusion Sinoaortic denervation and POA lesioned attenuated hypothermia induced by intraperitoneal injection of AVP, suggesting that sinoaortic baroreceptors and POA would involve in the hypothermic effect of peripheral vasopressin.
出处
《医学研究杂志》
2016年第3期70-73,共4页
Journal of Medical Research
基金
四川省教育厅科研基金资助项目(12ZB204)
四川省卫生厅科研基金资助项目(120495)
成都医学院学科建设项目专项基金资助项目(CYXK2012003)
