大鼠脊髓半切损伤后突触分化诱导基因产物SynDIG1的表达增强

Expression Enhancement of Syn DIG1 after Spinal Cord Hemisection Injury in Rats

目的探讨大鼠脊髓半切损伤后(spinal cord injury,SCI)突触分化诱导基因产物(synapse differentiation induced gene 1,Syn DIG1)的表达变化及意义。方法 SD大鼠随机分成对照组和脊髓损伤组(n=75),切断大鼠脊髓一半作为实验模型,只咬除椎板而不损伤大鼠脊髓作为对照模型。在手术后7、14、21、28、35天采集损伤区域的脊髓组织,用免疫荧光和免疫印迹技术检测各组各时间点Syn DIG1的蛋白表达,并且与半胱氨酸蛋白酶3(caspase-3)、神经生长蛋白43(GAP-43)等神经元特异性标志物做免疫荧光双标技术检测。结果在脊髓损伤后第7天就发现神经元有SynDIG1表达,在第28天达到高峰,随后维持在较高水平。通过免疫荧光双标技术发现脊髓损伤后Syn DIG1与GAP-43存在共表达。结论脊髓损伤后中期神经元大量表达Syn DIG1,激活了神经生长因子,促进神经元再生,有利于神经系统的修复。

Objective To observe expression and significance of SynDIG1 after spinal cord injury in rats. Methods SD rats were randomly divided into two groups（n=75）： in the injury group, the SCI was produced by hemisection of the cord at the 9 th thoracic level, and the sham operation rats were taken as the normal control. The animals were perfused at 7,14,21,28,35d after the operation, and the spinal cord was taken out at different time points. The levels of SynDIG1 expression were measured by immunofluorescence technique and Western blot. And the expressions of SynDIG1, GAP-43 and caspase-3 were detected using double immunofluorescent staining. Results The expression of SynDIG1 was found at 7d after the spinal cord injury, reached the peak at 28d after the injury. The double labeling results showed that on 14d after SCI, SynDIG1 and GAP-43 was detected in the injury group. Conclusion The high expression of SynDIG1 after spinal cord injury early, and activation of regeneration factors which resulting in the regenerate of nerve cells, is conducive to the repair of the nervous system.