自体骨髓间充质干细胞复合富血小板纤维蛋白促进新骨形成的研究

Research of Bone Marrow Mesenchymal Stem Cells Combined with Platelet Rich Fibrin Promoting New Bone Formation

目的研究自体骨髓间充质干细胞(bone marrow stem cells,BMSCs)复合富血小板纤维蛋白(platelet-rich fibrin,PRF)对兔拔牙窝骨缺损新骨形成的促进作用。方法将2月龄雄性新西兰兔27只,随机分3组,每组9只,建立动物模型,均在全身麻醉下微创拔除下颌左侧中切牙。A、B和C组分别为BMSCs复合PRF、单一PRF和空白对照。每组3只于3个时间点,即第4、8、12周,在骨缺损部位取材,拍摄X线片观察骨缺损区骨质的愈合及改建情况。同时制作HE染色的组织切片,进行骨计量观察。结果观察和使用灰度值统计软件进行分析的结果表明,在4、8、12周A组缺损区骨密度明显高于B、C组(P<0.01)。A组与B、C组的骨计量学比较分析显示骨小梁宽度、缺损区骨量、成骨细胞数差异均有统计学意义(P<0.01)。结论自体BMSCs复合PRF应用于兔牙槽骨缺损区能有效促进新骨形成。

Objective To study bone marrow stem cells （BMSCs） composited with platelet-rich fibrin （PRF） promote new bone formed on the defect of rabbit extraction sockets. Methods The 2-month-old male New Zealand rabbits with the number of twenty-seven were randomly divided among three groups, each nine, to establish the animal model. All were minimally invasive removal of mandibular incisor to the left under general anesthesia.The group A and B was implanted with BMSCs-PRF composite and single PRF respectively, and C was control.We removed a section of the bone defects from three rabbits of each group in four, eight and twelve weeks after operation respectively, and observed the bone healing and rebuilding using X-ray.Tissue sections stained with HE were prepared for histomorphometric observation. Results The results of observation and the use of statistical software gray value analysis showed that the bone density in defects of A group was significantly higher than that of B and C （P〈0.01）.By histomorphometric comparison, it indicated that trabecular width and bone defects as well as the number of osteoblast cells had significant differences between A groups and another two groups after the fourth, eighth, twelfth week （P〈0.01）. Conclusion Autologous BMSCs composites PRF applied to rabbit alveolar bone defects can effectively promote newbone formation.