多肉植物劳尔的组织培养

Tissue Culture of the Succulent Plant Sedum clavatum

以多肉植物劳尔(Sedum clavatum)叶片为外植体,通过诱导愈伤组织、愈伤组织分化新芽、芽生根和无菌苗移栽等步骤建立劳尔无菌苗快速繁殖体系。研究结果表明,诱导劳尔叶片愈伤组织的最佳培养基为MS+3.0 mg·L–1 6-BA+0.1mg·L–1 NAA+1 mg·L–1 KT,诱导率达95.7%;愈伤组织诱导分化新芽的最佳培养基为3/4MS+3.0 mg·L–1 6-BA+0.3 mg·L–1NAA,分化率达80%;芽生根的最佳培养基为1/2MS+0.03 mg·L–1 NAA,生根率可达94.89%;采用珍珠岩:蛭石(1:2)作为基质移栽培养生根苗,成活率达90%以上。实验成功建立了劳尔快速繁殖体系。

The leaf of Sedum clavatum was used to induce callus; through callus induction, bud induction, rooting and transplatation, aseptic-frequency regeneration systems were established. The optimal medium for induction was MS＋3.0 mg·L^-1 6-BA＋0.1 mg·L^-1 NAA＋1.0 mg·L^-1 KT; for redifferentiation 3/4MS＋3.0 mg·L^-1 6-BA＋0.3mg·L^-1 NAA; and for rooting 1/2MS＋0.03 mg·L^-1 NAA. The callus induction, redifferentiation and rooting rate was 95.7%, 80% and 94.89%, respectively. Using perlite：vermiculite（1：2） as a matrix, the transplant survival rate was 90%. We have established a high-frequency regeneration system for S. clavatum.