急性温度胁迫对银鲳幼鱼代谢酶、离子酶活性及血清离子浓度的影响

Effect of acute temperature stress on metabolic enzymes,ion enzymes and concentration of ion in serum of juvenile Pampus argenteus

为探明急性温度胁迫对银鲳(Pampus argenteus)幼鱼生化指标的影响,该实验设计了3个温度梯度,即22℃、27℃和32℃,以27℃为对照组,胁迫时间48 h,分别测定胁迫前后其代谢酶、离子酶活性以及血清中离子浓度。结果显示,2个实验组的血清谷丙转氨酶(GPT)的变化趋势相反,肝脏谷草转氨酶(GOT)的变化趋势也不同;2个实验组的碱性磷酸酶(AKP)变化趋势相反,而酸性磷酸酶(ACP)变化趋势相同,乳酸脱氢酶(LDH)在22℃实验组出现波浪式变化(P<0.05);血清钠(Na+)、钾(K+)、氯(Cl-)、钙(Ca2+)在各组呈现不同的变化趋势;鳃Na+/K+-ATP酶活性在32℃实验组出现下降趋势,肾脏Na+/K+-ATP酶活性在22℃实验组出现先升高后降低趋势(P<0.05);2个实验组鳃Ca2+/Mg2+-ATP酶活性和肾脏Ca2+/Mg2+-ATP酶活性皆出现相反的变化趋势。结果表明,急性温度胁迫可显著影响银鲳幼鱼的正常代谢,因此在养殖过程中应尽量避免温度胁迫,以使其有一个良好的生长环境。

To investigate the effect of acute temperature stress on biochemical indicators of juvenile Pampus argenteus, we designedtwo treatment groups （22 ℃ and 32 ℃ ） and a control group （27 ℃ ） with stress time of 42 h. The GPT in serum and GOT in liver intwo experimental groups changed oppositely; AKP varied in an opposite manner but ACP changed in the same way; LDH in 22 ℃group changed in a wave-like manner （ P 〈 0. 05 ）. Na ＋ , K ＋ , C1- and Ca2＋ concentrations in three groups changed differently. TheNa ＋/K ＋ -ATP activity in gill in 32 ℃ group decreased, but that in kidney in 22 ℃ group increased first then decreased （P 〈 0. 05 ）.In two experimental groups, Ca2 ＋/Mg2 ＋ -ATPase activities in gill and kidney varied in oppositely （ P 〈 0. 05）. It is suggested that a-cute temperature stress, which should be avoided in breeding for juvenile P. argenteus, affects normal metabolism of the juveniles sig-nificantly.