叶下珠甲醇提取物抗炎镇痛及体外抑菌作用研究

Study on Analgesic and Anti-Inflammatory Effects in Methanol Extracts of Phyllanthus urinaria L.and Its Effect on Bacteria in vitro

目的:研究叶下珠甲醇提取物的抗炎镇痛及体外抑菌作用,为临床用药提供科学的理论依据。方法:采用二甲苯致耳肿胀实验、冰醋酸致小鼠扭体反应实验、冰醋酸致小鼠腹腔毛细血管通透性增加实验及福尔马林致痛实验,观察叶下珠甲醇提取物高剂量组(18 g/kg)、中剂量组(9 g/kg)、低剂量组(4.5 g/kg)的抗炎镇痛作用。采用K-B纸片扩散法研究叶下珠甲醇提取物对金黄色葡萄球菌和大肠杆菌的体外抑菌作用。结果:叶下珠甲醇提取物高、中、低剂量组均可明显减轻小鼠耳廓肿胀度(P<0.05或0.01)、明显抑制小鼠腹腔毛细血管通透性增加(P<0.05或0.01)、明显减少冰醋酸致小鼠扭体反应次数(P<0.01),叶下珠甲醇提取物高、中、低剂量组均可显著降低福尔马林致痛实验的第Ⅰ时相的疼痛强度(P<0.05或0.01),高剂量组对第Ⅱ时相的疼痛强度降低的程度有显著性差异(P<0.05)。叶下珠甲醇提取物对金黄色葡萄球菌和大肠杆菌的抑菌率分别为82.08%、59.36%。结论:叶下珠甲醇提取物具有较好的抗炎镇痛作用,对金黄色葡萄球菌和大肠杆菌具有体外抑菌作用。

Objective： To investigate the analgesic and anti-inflammatory effects in methanol extracts of Phyllanthus urinaria L.and its effect on bacteria in vitro and to provide scientific experiment evidence for clinical applications.Methods： The analgesic and anti-inflammatory effects were observed by using pain models induced by the ear edema of mice induced by xylene,capillary permeability in abdominal cavity of mice,twisting reaction of mice induced by acetic acid and Formalin test.At the same time,different doses were used.Bacteriostatic activities of the methanol extracts of Phyllanthus urinaria L.were tested by paper disc diffusion method.Results： The methanol extracts of Phyllanthus urinaria L.could effectively inhibit capillary permeability（ P〈0.05 or P〈0.01） and lighten ear edema in mice model（ P〈0.05 or P〈0.01） and decrease the twisting reaction（ P〈0.01） and the ache degree of mice induced by formalin in the first phase（ P〈0.05 or P〈0.01）.There was significant difference of the pain intensity decrease in the high dose group at the second phase（ P〈0.05）.The methanol extracts of Phyllanthus urinaria L.have different degrees of inhibition on Staphylococcus aureus and Escherichia coli and the antibacterial rate reached to 89.67% and 66.00%.Conclusion： The methanol extracts of Phyllanthus urinaria L.have significant effects on analgesia and anti-inflammation and against staphylococcus aureus and Escherichia coli in vitro.