H-1细小病毒抗体ELISA检测方法的建立与应用

Establishment and application of ELISA method for H-1 parvovirus

目的建立H-1细小病毒抗体的ELISA检测方法,并进行初步应用。方法采用大鼠神经胶质瘤细胞C6培养大鼠H-1细小病毒,制备包被抗原,采用纯化后抗原建立该病毒的ELISA检测方法;将建立的方法与国外同类试剂盒进行比对,考察该方法的特异性和灵敏度。同时,应用该方法对35份大鼠血清进行检测。结果所建立的方法可检测出稀释1280倍的阳性血清;与犬细小病毒、小鼠微小病毒和猪细小病毒阳性血清均无交叉反应;与大鼠细小KRV病毒有交叉反应;对35份大鼠血清进行检测,结果均为阴性,与国外同类试剂盒结果一致。结论所建立的H-1细小病毒ELISA检测方法具有良好的种属特异性和灵敏度,可用于大鼠血清中H-1细小病毒抗体检测。

Objective To establish ELISA method for H-1 parvovirus,and to apply it in detection. Method Cultured the H-1 parvovirus in rat glioma cell line C6,prepared the viral antigen for coating. Used the purified viral antigen to establish the ELISA method,and compared the ELISA method with the ELISA kit from Xpress Bio company.Then applied the ELISA method in detection of 35 rat serums. Results The positive serum which be diluted to 1280 can be detected by the ELISA method,there have not cross reaction with positive serum of CPV,MVM and PPV,but there has cross reaction with KRV. 35 pieces of rat serums were detected by the ELISA method,they were all negative,the results were consistent with the kit from Xpress Bio company. Conclusions The sensitivity and species specificity of the ELISA method for H-1 parvovirus were suitable,the method can be used in detection of H-1 parvovirus in rat serum.