联合使用甲状旁腺激素和辛伐他汀对去卵巢大鼠骨质疏松症的治疗有叠加效应

Combined treatment with parathyroid hormone (1-34) and simvastatin had an additive effecton osteoporosis healing in OVX rats

目的探索联合使用甲状旁腺激素(PTH)和辛伐他汀(SIM)对去势大鼠骨质疏松的防治作用。方法 50只健康雌性SD大鼠随机行假手术(Sham,N=10)和切除双侧卵巢(OVX,N=40)手术后,OVX大鼠随机的分成4组:OVX组、SIM组、PTH组、PTH+SIM组。术后第1天开始给予药物治疗,SIM组:SIM灌胃(剂量5 mg/kg,每天1次),PTH组:PTH皮下注射(剂量60μg/kg,每周3次),PTH+SIM组:SIM灌胃和PTH皮下注射,剂量和用药频率和SIM组、PTH组相同,直至手术后12周为止,12时所有大鼠处死取胫骨行Micro-CT检测。结果结果表明SIM组、PTH组、PTH+SIM组和OVX组相比,胫骨近端都有较高的BMD、BV/TV、Tb.Th、Tb.N、Conn.D和较低的Tb.Sp,其中PTH+SIM组大鼠胫骨近端有最高的BMD、BV/TV、Tb.Th、Tb.N、Conn.D和最低Tb.Sp。SIM和PTH单独使用的效果明显低于他们联合使用对去卵巢大鼠骨质疏松的防治作用。

Objective This study was designed to investigate the impact of combined treatment with Simvastatin（ SIM） and Parathyroid hormone（ PTH） on osteoporosis healing in OVX rats.Me thods After bilateral ovariectomy（ OVX,N = 40） and Sham-operation（ Sham,N = 10）,then all animals from OVX group randomly divided into four groups：group OVX,group SIM,group PTH and group PTH ＋ SIMand received NS,SIM（ 5 mg / kg,daily）,PTH（ 60 μg / kg,three times a week）,or PTH plus SIMuntil death 12 weeks.The distal femurs of rats were harvested for evaluation.The results of treatment for osteoporosis were evaluated by micro-computerized tomography.Re sults Comparison with group OVX,the tibia from groups SIM,PTH and PTH＋ SIMhave a higher BMD、BV / TV、Tb.Th、Tb.N 、Conn.D and a lower Tb.Sp.PTH ＋ SIMproduced strongest effects on BV /TV,Tb.Th,Tb.N,Conn.D,BMD and Tb.Sp.The effect of SIMand PTH using alone is significantly lower than their combined using for prevention and treatment of osteoporosis to ovariectomized rats.Conclusion These results seemed to indicate that PTH plus SIMhad an additive effect on osteoporosis in OVX rat.