两株海洋极端生境微生物防治番茄灰霉及促进抗盐抗寒作用研究

Effects of Two Marine Microorganisms on Tomato Leaf Mold and Their Resistance to Salt and Cold

采用抗生素标记研究了来自深海环境菌株NJ-01和KG89在番茄的定殖及对灰霉病的防治,采用隶属函数值和防御酶活性等综合评价了其诱导植物的抗盐、抗寒作用。菌株NJ-01和KG89均能在番茄根、茎、叶内部定殖,在根部的定殖数量分别为6.50×103和4.90×103 cfu/m L;对番茄灰霉病具有较高的防效,可达87.94%和74.55%,同时,菌株提高植物防御酶SOD和POD等活性122.22%~234.33%,提高植物体内脯氨酸含量增加133.09%~149.37%,可溶性糖的含量68.53%,电解质外渗率和脂质过氧化产物含量减少36.45%~63.12%,达到提高番茄的抗盐及抗寒能力。

We investigated the colonization and the effects of marine microorganisms NJ-01 and KG89 marked by antibiotics on leaf mold disease（Botrytis cinerea） resistance, salt and cold tolerance and defense enzymes activities of tomato plants. Results showed that the two strains could well colonize in roots, stems and leaves of tomatoes. Prevention efficacy against mold disease were 87.94% and 74.55% by NJ-01 and KG89, respectively. The two strains could improve salt and cold tolerance of tomato plants by increasing SOD and POD activities by 122.22%—234.33%, proline content by 133.09%—149.37%, soluble sugar content by 68.53%, and reducing the relative electric conductivity and MDA content by 36.45%—63.12%.