崂山常见中药材提取物对烟草青枯病菌的抑制作用

Antibacterial Activities of Extracts from Medicinal Herbs in Laoshan Mountain against Ralstonia solanacearum

以烟草青枯病菌Ralstonia solanacearum为病原指示菌,从艾叶Artemisiae argyi等40种崂山常见中药材95%乙醇提取液中筛选对其具有明显抑制作用的活性物质。结果表明,32种(80%)药材提取物显示出不同程度的抑菌作用,其中公丁香Syzygium aromaticum提取物抑菌活性最强,其抑菌圈直径达19.53 mm。采用气相色谱-质谱(GC-MS)联用法分析鉴定了公丁香提取物中12种化学组分,其中相对含量最高的丁香酚(40.43%)抑菌活性最强,抑菌圈直径达18.50 mm,处理6 h对病菌的抑制中质量浓度(EC50)值为0.0292 mg/m L。本研究表明崂山地区抑菌植物具有丰富的多样性,公丁香提取物中活性成分丁香酚具有防治烟草青枯病的良好应用潜力。

Ethanol extracts of 40 medicinal herbs were evaluated for their antibacterial activities against Ralstonia solanacearum and the active ingredients of extract exhibiting optimal antibacterial effect was analyzed. The results indicated that ethanol extracts of 32（80%） species showed various antibacterial activities. The inhibition rate of extract of clove（Syzygium aromaticum） was the most pronounced with the inhibition zone diameter of 19.53 mm.Gas chromatography mass spectrometry（GC-MS） analysis revealed a total of 12 chemical constituents in the extract of clove. The major constituent eugenol with the relative content of 40.43% exhibited the highest antibacterial activity with the inhibition zone diameter of 18.50 mm and the EC50 value of 0.0292 mg/m L at 6 h after treatment. Our study suggests that plant species with antibacterial activities in Laoshan mountain have a big variety and the active ingredient eugenol of clove extract could be a potential biocontrol agent to control tobacco bacterial wilt.