奇魅植物酵素对小鼠酒精性肝损伤保护作用的研究

Protection of Qimei Fermented Beverage against alcohol-induced liver injury in mice

目的探讨奇魅植物酵素对小鼠酒精性肝损伤的保护作用。方法用连续灌服酒精的方法建立小鼠酒精性肝损伤模型,将60只雄性昆明小鼠分为5组:空白对照组,模型组,低剂量组、中剂量组、高剂量组(模型组基础上分别加5、25、50mL/kg奇魅植物酵素)。连续灌胃9周后,测定各组小鼠血清中天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)的活性。测定各组小鼠肝组织中丙二醛(MDA)、三酰甘油(TG)、还原性谷胱甘肽(GSH)、乙醇脱氢酶(ADH)及乙醛脱氢酶(ALDH)的活性,以及肝指数的变化及肝组织病理学观察。结果成功建立小鼠酒精性肝损伤模型;与模型组比较,各剂量组的奇魅植物酵素均可有效地降低血清中AST、ALT及肝组织中TG水平,提高ADH、ALDH、GSH水平。此外,病理学观察结果与酶学变化相一致。结论奇魅植物酵素对小鼠酒精性肝损伤有一定的保护作用。

Objective To explore the protective effect of Qimei Fermented Beverage against alcohol-induced liver injury in mice.Methods Alcohol-induced liver injury mouse model was established by continuously intragastric administration.Totally 60 male mice were randomly divided into five groups,blank control group,model group,low,middle and high doses groups（adding 5,25,50mL/kg Qimei Fermented Beverage）.After continuous intragastric administration for 9weeks,the levels of serum aspartate transaminases（AST）and alanine transaminases（ALT）were detected in each group,moreover the levels of malondialdehyde（MDA）,triglyceride（TG）,glutathione（GSH）and alcohol dehydrogenase（ADH）,aldehyde dehydrogenase（ALDH）of liver homogenate,and liver index change were measured.Liver histopathological changes were observed.Results The alcohol-induced liver injury model in mice was successfully established.Compared wit model group,different doses groups of Qimei Fermented Beverage could effectively reduce the levels of serum AST,ALT and liver tissue TG,increased the levels of ADH,ALDH,GSH.In addition,the pathological examination results of liver were consistent with those in enzymology.Conclusion Qimei Fermented Beverage has certain protective effect against alcohol-induced liver injury and can obviously improve the alcohol-induced liver injury.